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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 16, 2012 to October 17, 2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study (DIN 38412) with acceptable restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report date:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, Part 11 (October 1982)
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
EC Number:
213-485-0
EC Name:
3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
Cas Number:
957-68-6
Molecular formula:
C10H12N2O5S
IUPAC Name:
(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Name: 7-Aminocefalosporansäure (7-ACA)
Lot no.: B146988

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Sample volume in the reaction flask: 20 ml samples in 50 ml flasks, for each sample 10 animals were tested,

Test solutions

Details on test solutions:
Stock solution: 0.6 g/L (stored for 21 days at 5°C)
TOC of stock solution: 241 mg/l [C]
concentration of the stock solution: 557 mg/l (calculated from TOC and Purity)

Concentration in the flasks: 75, 107, 150, 214, 300, 426 and 557 mg/l

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
not specified
Water media type:
not specified

Test conditions

Reference substance (positive control):
yes
Remarks:
K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
119 mg/L
Nominal / measured:
estimated
Conc. based on:
other: calculation
Basis for effect:
mobility
Remarks on result:
other: VB for p=95%: 96.7 - 132.2 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
155 mg/L
Nominal / measured:
estimated
Conc. based on:
other: calculation
Basis for effect:
mobility
Remarks on result:
other: VB for p=95%: 141.2 - 171.3 mg/L
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
EC50: 0.93 mg/L, VB for p=95%: 0.649 - 1.132 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The test went flawlessly. No animal of the control samples withaout test item was immobile at the end of the test. The EC50 value of the reference substance was between 0.9 and 1.9 mg/L.
Conclusions:
The EC50 value of the test item is 155 mg/L.