Registration Dossier
Registration Dossier
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EC number: 213-485-0 | CAS number: 957-68-6
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 06, 1996 to January 27, 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study (OECD 209) with acceptable restrictions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- 102822-64-0
- Cas Number:
- 102822-64-0
- IUPAC Name:
- 102822-64-0
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Name: G-7-ACS Mono-Na-Salz
Lot: Op 1/96 vom 28.02.1996
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Stock solution:
Amount of test item: 2.00 g in 700 ml very clean water
Stirring time: 16.5 hours
Temperature: 23-25°C
pH value after stirring: 3.7
pH was adjusted to 5.6
The clear solution was filled up to 1000 mL.
Concentration of the stock solution: 2000 mg/L
Amount of stock solution used for the test: 500 ml/L
Highest testing concentration 1000 mg/L
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Source: Sewage cleaning plant of the city Frankfurt am Main in Sindlingen
Type: washed activated sludge of a communal sewage plant
Test amount: 450 ml/L sludge suspension
Solid content: 3.56 g/L (dry substance)
Content in the end test volume: 1.6 g/L (dry substance)
Acclimatization status: Not acclimatized to the test item and reference substance
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20.5-21.0°C
- pH:
- pH of the culture solution: 7.1-7.3 before incubation
- Dissolved oxygen:
- 5.3-5.4mg O2/L
- Nominal and measured concentrations:
- nominal 1000 mg/L and measured 992.2 mg/L at start of the test and measured 987.9 mg/L at end of the test
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol (5, 20 and 30 mg/L)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 d
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 d
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- From the measured inhibition values the EC values could be determined. Up to the highest test concentration no significent inhibition occurred.
- Results with reference substance (positive control):
- EC50 of 3,5-Dichlorphenol: 11.8 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Up to the highest test concentration of 1000 mg/L no significent inhibition occurred. Therefore all EC values, EC20, EC50 and EC80 are above 1000 mg/L.
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