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EC number: 220-813-6 | CAS number: 2905-62-6
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test material was not readily biodegradable under the conditions of the headspace test performed.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of the test material was investigated in a headspace test conducted in accordance with the standardised guidelines OECD 310 and ISO 14593 under GLP conditions. The study has been assigned a reliability score of 1 in line with the principles for assessing data quality as defined by Klimisch et al. (1997).
Activated sludge from a municipal sewage treatment plant was exposed to the test material for 28 days at an organic carbon concentration of 20 mg C/L. The test material was incubated in an inoculated mineral salts medium in well sealed glass serum bottles with a headspace of air.
The test consisted of five groups: Inoculum blank (inoculated mineral medium), procedure control (inoculated mineral medium, reference material (1-octanol) at 20 mg C/L), test material (inoculated mineral and test material at 20 mg C/L), toxicity control (inoculated mineral medium and test and reference material at 20 mg C/L) and abiotic control (untreated mineral medium and test material at 20 mg C/L).
The CO2 evolution resulting from the aerobic biodegradation of the test material was determined by measuring the inorganic carbon (IC) produced in the test bottles in excess of that produced in blank vessels containing inoculated medium only. Biodegradation was expressed as a percentage of the theoretical maximum IC production, based on the quantity of test material (as organic carbon) initially added.
The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of the test material.
In the toxicity control, the test material was found not to inhibit microbial activity. In the abiotic control no significant mass of inorganic carbon, indicating biodegradation, was observed. Since all criteria for acceptability of the test were met, this study was considered to be valid.
The test material was not readily biodegradable under the conditions of the headspace test performed.
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