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Toxicity to soil macroorganisms except arthropods

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Description of key information

NOEC (14 d) ≥ 1000 mg/kg dw for Eisenia fetida (OECD 207)

Key value for chemical safety assessment

Additional information

No experimental data evaluating the toxicity of 1,2,3-propanetriyl trioleate (CAS No. 122-32-7) to soil macroorganisms are available. Therefore, toxicity data from a structurally related analogue substance (Glycerides, C14-18 and C16-18 unsatd. mono-, di- and tri- (CAS No. 91052-28-7) are used as read-across in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Both substances are UVCBs, formed from the esterification of fatty acids and glycerol, having a high content (>60%) of C18 unsaturated fatty acid and to a certain extent C16, C14 and C12 fatty acids. The log Kow values of these substances (> 5 and > 4.6 for 1,2,3-propanetriyl trioleate and Glycerides, C14-18 and C16-18 unsatd. mono-, di- and tri-, respectively) indicate a high adsorption potential to soil particles. Furthermore, their water solubility and aquatic toxicity profiles are comparable (see Aquatic toxicity section). Based on the above information, the use of data from Glycerides, C14-18 and C16-18 unsatd. mono-, di- and tri- as read-across for 1,2,3-propanetriyl trioleate is considered justified.


The toxicity of Glycerides, C14-18 and C16-18 unsaturated, mono-, di- and tri- (CAS No. 91052-28-7) to earthworms was evaluated by Moser (2012). Eisenia fetida was exposed to the test substance for 14 days at a concentration of 1000 mg/kg dw (limit test). Due to the poor solubility of the substance in water, test solutions were prepared using a solvent. No effects on survival or biomass during the exposure period were reported, leading to a NOEC (14 d) ≥ 1000 mg/kg dw.


1,2,3-propanetriyl trioleate is readily biodegradable. Therefore, rapid and ultimate degradation in the environment, including soil, can be expected. Chronic exposure of terrestrial organisms is thus very unlikely. Furthermore, this substance did not show acute or chronic toxicity to aquatic organisms. These results indicate no toxicity to terrestrial organisms is expected either. Due to the metabolization via enzymatic hydrolysis of the Glycerides category members, a relevant uptake and bioaccumulation in biota is not expected. Enzymatic breakdown will initially lead to the free fatty acid and glycerol. Glycerides are naturally stored by organisms as long-term energy reserves. Especially in periods in which the energy demand is high (reproduction, migration, etc.), glycerides are mobilized from the storage sites as source of fatty acids. Fatty acid catabolism is the most important energy source in many species, resulting in the release of acetyl CoA and NADH (throughβ-oxidation) and eventually, via the tricarboxylic cycle, the production of metabolic energy in the form of ATP. Please refer to IUCLID Section 5.3.1 for a detailed overview on bioaccumulation of the Glycerides category members.

Based on the results obtained for the structurally related analogue (in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5) and the characteristics of 1,2,3-propanetriyl trioleate (CAS No. 122-32-7), this substance is not expected to show toxicity to soil macroorganisms.