methyl (2E)-[2-(chloromethyl)phenyl](methoxyimino)acetate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): 520 F - Chlormethyloximether
- Physical state: solid/ dark green
- Analytical purity: 96.8 ± 0.1%
- Batch No.: 31196-182
- Storage condition of test material: ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution (10 mg/L) was prepared daily by dissolving 20 mg of test susbtance in 2 L of test medium and treated by ultra sonification for approx. 20 min to ensure that the test substance was completely dissolved. The test concentrations were prepared by dilution of this stock solution. Before the dilution was performed, the stock solution was checked for complete dissolution of the substance. All stock solutions appeared colorless-clear.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Feeding during test: yes
- Food type: unicellular green algae (Desmodesmus subspicatus)
- Amount: increasing amount
- Frequency: daily

CULTURE CONDITIONS
- Photoperiod: 16 h light, 8 h darkness
- Temperature: 20 ± 1 °C
- Diet: Unicellular algae Desmodesmus substicatus
- Feeding: daily
- Renewal of culture water: twice weekly

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

2.48 - 2.50 mmol/L

Test temperature:

19 - 20 °C

pH:

7.9 - 8.2

Dissolved oxygen:

8.9 - 9.2 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 0.32, 0.56, 1.0, 1.8, 3.2 mg/L
Time-weighted mean measured concentrations: 0, 0.243, 0.442, 0.777, 1.387, 2.464 mg/L
Recovery rates: -, 76, 79, 78, 77, 77%

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: glass beakers covered with glass petri plates to slow evapuration, 100 ml, 50 ml
- Aeration: none
- Renewal rate of test solution: daily
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 0.02 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (Elendt M4)
- Intervals of water quality measurement: temperature, oxygen content, pH and hardness at day 0, 8, 14 in the freshly prepared test solutions, and at day 1, 9, 15 before renewal in the old test solutions

OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h darkness
- Light intensity: about 640 - 679 lux at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED: daily measurements of young production , mortality of the parent animals and young, and presence of aborted eggs

RANGE-FINDING STUDY
- Results used to determine the conditions for the definitive study: EC0reproduction (17 d) = 2 mg/L; LC100 (9 d) was between 2 and 6.25 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.442 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.777 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.442 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

(length)

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.777 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

(length)

Effect concentrations 5

Duration:

21 d

Dose descriptor:

other: EC0

Effect conc.:

0.777 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 6

Duration:

21 d

Dose descriptor:

other: EC20

Effect conc.:

1.766 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 7

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

3.268 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 8

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 2.464 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality