Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 464-520-3 | CAS number: 189813-45-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): 520 F - Chlormethyloximeter
- Physical state: solid/ dark green
- Analytical purity: 94.4 g / 100 g
- Batch No.: 31196-182
- Storage condition of test material: ambient temperature
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance is not stable in water and the dissolvation is slow. The test concentrations were prepared without a prolonged stirring period to avoid hydrolysis before start of exposure. Undissolved test substance was not removed, so that the dissolvation process could proceed. The test results can be attributed to the test substance and to the hydrolysis products that were not analysed. For preparing the test solutions the test substance was stirred for approx. 1 hour in the test water. Each test concentration was prepared separately.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Hatch at the laboratory for Experimental Toxicology and Ecology
- Age at study initiation: 14 months
- Length at study initiation (length definition, mean, range): from the top of the snout to the end of the caudal fin, 3.6 cm (3.0 – 4.3 cm)
- Weight at study initiation (wet weight): 0.45 g (0.22 – 0.94 g)
- Feeding during test: none
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Photoperiod: 16 hours light, 8 hours dark
- Type and amount of food: Commercial fish diet „Tetramin“, supplier Tetra-Werke, Melle, Germany, ad libitum, additionally generally on workdays live brine shrimp (artemia).
- Health during acclimation: no signs for sickness, injuries or abnormalities were observed
- Mortality during the last week before start of exposure: 0 %
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 100 mg/L CaCO3
- Test temperature:
- 23 °C
- pH:
- 8.0 - 8.4
- Dissolved oxygen:
- 8.1 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: control, 10, 22, 50, 100, 150 mg/L
Mean measured concentrations: -, 3.18, 6.18, 13.5, 45.3, 63.3 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, fill volume: Glass aquaria, 30 cm long, 22 cm wide, 24 cm high, 10 L
- Aeration: slight
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 7
- No. of vessels per control (replicates): 7
- Biomass loading rate: 0.32
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Non chlorinated charcoal filtered tap water mixed with deionized water, aerated
- Total hardness: Approx. 1 mmol/L = 100 mg/L CaCO3
- Conductivity: Approx. 250 μS/cm (at 25°C)
- Ca content: Approx. 40 mg/L
- Mg content: Approx. 5 mg/L
- Acid capacity: Approx. 2.5 mmol/L
- pH-value: Generally 7.5 – 8.5
- Intervals of water quality measurement: Temperature, oxygen content and pH-value were measured in each test vessel within 1 hour after start of exposure and after 24, 48, 72 and 96 hours
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: Approx. 36 - 191 Lux (the light intensity is determined in regular intervals at the surface of the aquaria)
EFFECT PARAMETERS MEASURED: Survival and toxic signs (changes appearance, swimming behaviour and behaviour in comparison to the control group) within 1 hour after start of exposure and after 4, 24, 48, 72, 96 hours
TEST CONCENTRATIONS
- Range finding study: yes (non GLP)
- Test concentrations: LC50 > 100 mg/L
- Results used to determine the conditions for the definitive study: The highest test concentration 150 mg/L was selected because of the instability of the test substance
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 96.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - Mortality of control: none
- Behavioural abnormalities:
- 50 mg/L treatment group: 4 fish were tottering after 1 and 4 hours and 2 fish after 24 hours
- 100 mg/L treatment group: 2 fish were tottering after 1 hour and 7 fish after 4 and 24 hours; 5 fish were swimming at the bottom after 1 hour, 3 fish after 72 hours and 2 fish after 96 hours (the remaining fish were dead)
- 150 ml treatment group: 7 fish were tottering and swimming at the bottom after 1 hour; 2 fish showed apathy after 4 hours
- Dissolution behaviour: homogeneous dispersion of the test substance in water, turbidity; undissolved test substance at the bottom of the test vessel and undissolved test substance on the water surface in the 50, 100 and 150 mg/L treatment groups
Any other information on results incl. tables
- Sublethal observations / clinical signs:
The effects observed in this study were the combined effects of the test substance and its products of hydrolysis, which were not analysed. Therefore the evaluation can be based on the nominal concentrations of the test substance.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.