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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 DEC 2000 - 5 FEB 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17).The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 301C.
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
>= 28 d
Initial conc.:
300 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
according to guideline
Reference substance:
aniline
Test performance:
The reference substance showed 77 % degradation after day 21 of the experiment and later.
Parameter:
% degradation (test mat. analysis)
Value:
2
Sampling time:
28 d
Details on results:
The test substance showed 2 % degradation at day 28 of the experiment.
Parameter:
COD
Value:
100.5 mg O2/g test mat.
Results with reference substance:
The reference substance showed 77 % degradation after day 21 of the experiment and later.
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The test item was found to be NOT readily biodegradable under the described conditions.
Executive summary:

Purpose

The purpose of this assay was to identify the biodegradation potential of the registered substance in aquatic environments to provide a rational basis for environmental fate estimation for the test item.

Study Design

The test item was investigated for its ready biodegradability in a MITI test over a period of 28 days (OECD Guideline 301C, GLP). The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.

Result

Biodegradation of the test material:

After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period, degradation rates of 2 % and 3 % were found.

Biodegradation of Aniline

The reference item Aniline was sufficiently degraded to 77 % after 21 days and thereafter, thus confirming the suitability of the used activated sludge inoculum.

Conclusion

The test material was found to be not ready biodegradable under the test conditions within 28 days.

Description of key information

Key, 28 d, OECD 301C, GLP: not ready biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information

Purpose

The purpose of this assay was to identify the biodegradation potential of the registered substance in aquatic environments to provide a rational basis for environmental fate estimation for the test item.

Study Design

The test item was investigated for its ready biodegradability in a MITI test over a period of 28 days (OECD Guideline 301C, GLP). The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.

Result

Biodegradation of the test material:

After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period, degradation rates of 2 % and 3 % were found.

Biodegradation of Aniline

The reference item Aniline was sufficiently degraded to 77 % after 21 days and thereafter, thus confirming the suitability of the used activated sludge inoculum.

Conclusion

The test material was found to be not ready biodegradable under the test conditions within 28 days.