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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 MAR 2001 - 05 JUN 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
This study was conducted according to Good Laboratory Practice (GLP) and the method applied follows that described in the OECD Guideline for Testing of Chemicals 305 (Flow through fish test).
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
GLP compliance:
yes (incl. certificate)
Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: days 3, 11, 18, 32, 46, 60
- Sampling intervals/frequency for test medium samples: days 3, 11, 18, 32, 46, 60
- Fish: two fish per sampling homogenised, and extracted with THF. Further analysis performed by GC-MS.
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
HCO-40, 5000 mg/L
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Length: 8.2 - 11.2 cm
Batch: 866-0024
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
60 d
Hardness:
100 mg/L
Test temperature:
25
pH:
7.7-7.9
TOC:
0.9
Details on test conditions:
according to guideline
Nominal and measured concentrations:
0.02 mg/L
0.002 mg/L
Regular concentration control analysis of the test media showed that the test material concentrations were maintained at about 95 % of the nominal values.
Details on estimation of bioconcentration:
HPLC
Conc. / dose:
0.02 mg/L
Temp.:
25 °C
pH:
7.8
Type:
BCF
Value:
78 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
18 d
Calculation basis:
steady state
Conc. / dose:
0.002 mg/L
Temp.:
25 °C
pH:
7.8
Type:
BCF
Value:
290 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
18 d
Calculation basis:
steady state
Validity criteria fulfilled:
yes
Conclusions:
The mean BCF for the test material as determined in a flow through test system amounts to 184 in this test system.
Executive summary:

Purpose

The purpose of this study was to determine the biocencentration potential of the test material using Cyprinus carpio in a flow through assay to provide a rational basis for hazard estimation for the test item in aquatic environments.

Study Design

The bioconcentration factor of the test substance to Cyprinus carpio was determined in a flow through test according to OECD 305.

Results

During the test no mortality was observed. Given test concentrations of the test material in the aquatic media of 20 µg/L and 2 µg/L yield BCFs of 78 and 290. From these data, a mean BCF of 184 can be calculated.

Conclusion

The mean BCF for the test material as determined in a flow through test system ammounts to 184 in this test system.

Description of key information

Key, Cyprinus carpio, 60 d, OECD 305, GLP, BCF at 0.02 mg/L = 290,  BCF at 0.002 mg/L = 78, mean BCF = 184

Key value for chemical safety assessment

BCF (aquatic species):
184 dimensionless

Additional information

Bioaccumulation was tested in fish (Cyprinus carpio) exposed for 60 days to the test material at concentrations of 0.02 and 0.002 mg/L in a flow through system. Regular concentration control analysis of the test media showed that the test material concentrations were maintained at about 95 % of the nominal values, throughout the exposure period and that the test material was present in dissolved form, thus proving steady state exposure conditions. No signs of toxicity were observed throughout the study. Steady state BCFs of 290 and 78 (mean 184) were determined. Due to the low bioaccumulation potential, no depuration phase was required. Based on these data it is concluded that the test material showed no relevant bioaccumulation (BCF < 2000).