Methyl 2-((allyloxy)methyl)acrylate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 Apr 2013 - 13 Jun 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

methyl 2-[(prop-2-en-1-yloxy)methyl]prop-2-enoate

EC Number:

819-688-6

Cas Number:

219828-90-7

Molecular formula:

C8H12O3

IUPAC Name:

methyl 2-[(prop-2-en-1-yloxy)methyl]prop-2-enoate

Test material form:

liquid

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

female

Details on test animals or test system and environmental conditions:

- Supplier : Orient Bio Co., Ltd. 699-13, Mokdong-ri, Buk-myeon, Gapyeong-gun, Gyeonggi-do, Korea
- Number of animals and sex distinction at the time of receipt : 14 females
- A range of age and body weight at the time of receipt : 8 weeks old, 174.8 g ~ 186.2 g
- A range of age and body weight at the time of administration : 9 weeks old, 180.0g ~ 182.8 g (1st step), 181.7 g ~ 188.2 g (2nd step), 10 weeks old, 190.3 g ~ 197.1 g (3rd step)


- Housing: stainless stell cage
- Diet:ad libitum
- Water: ad libitum
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3°C
- Humidity (%): 50 ±2 0
- Air changes (per hr): 10 - 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 30 mg/mL and 200 mg/mL
- Amount of vehicle (if gavage): 10 mL/kg bw)

MAXIMUM DOSE VOLUME APPLIED: 2000 mg/kg bw

Doses:

300, and 2000 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 4 hours after treatment and then daily for 14 days
- Frequency of observations and weighing: Body weight was measured at animal receipt day, animal allocation day, just before treatment and on day 7 and 14 after the administration. Body weight of dead animal was also measured when it was found to be dead.
- Necropsy of survivors performed: yes

Results and discussion

Preliminary study:

not specified

Effect levels

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

> 300 - < 2 000 mg/kg bw

Based on:

test mat.

Mortality:

All the animals died in 3rd step(2000 mg/kg body weight), two (Animal No. : 2302, 2303) animals on day 1 and one (Animal No. : 2301) animal on day 2 after adminstration. No mortality was observed in the 1st and 2nd step (300 mg/kg body weight) groups.

Clinical signs:

other: Clinical signs related with the substance, the salivation, inanimation and prone position, were observed from 2 hour to day 1 in 3rd step (2000 mg/kg) dose group. No clinical signs was observed in 1st and 2nd step (300 mg/kg) groups. Clinical signs were c

Gross pathology:

In necropsy finding of died animals, test substance retention in the stomach were observed in all (Animal No. : 2301 ~ 2303) died animals. There were no necropsy findings caused by administration of test substance in all survived animals.

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

Based on the results, the 2-(Allyloxymethyl) acrylic acid methyl ester was classified in to GHS (Globally Harmonized Classification System for Chemical Substances and Mixtures) Category 4 (300 mg/kg body weight < LD50 < 2000 mg/kg body weight) in this study.