Methyl 2-((allyloxy)methyl)acrylate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 Dec 2013 - 30 Jan 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
range finding test - 1, 10, 100 mg/L,
main test - 1, 1.8, 3.2, 5.6, 10mg/L
- Sampling method: one set of new sample solution at time of preparation and one at end of exposure.
- Sample storage conditions before analysis: frozen

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test solutions, approximately 100mL, were poured into each of the five vessels per test group and immediately plugged with a ground-in stopper to prevent volatilization of the test substance.
One of the five vessels of each test group was used for measuring the test conditions.

Test organisms (species):

Daphnia magna

Details on test organisms:

Source: National Institute for Environmental Studies, Japan
Growth stage: juveniles less than 24-hour old (female)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

242 mg CaCO3/L

Test temperature:

20 ± 1°C

pH:

8.5

Dissolved oxygen:

not specified

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

Nnominal: 1, 1.8, 3.2, 5.6, 10 mg/L
Mean measured: 0.951, 1.55, 3.05, 5.49 and 9.87 mg/L

Details on test conditions:

Duration: 48 hours
Exposure procedure: static,
Volume of the test solutions:100mL/vessel
Number of vessels: 4 vessels/ test group
Loading: 20 daphnids/test group (5 daphnids/vessel)
Temperature: 20 ± 1°C
pH: 6.0 - 9.0
Light: Fluorescent light, 16 hour light (800 lux or less)/ 8 hour dark
Feeding: None

RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

8.22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.94 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

not specified

Validity criteria fulfilled:

yes

Conclusions:

48 hour EC50: 3.94mg/L
48 hour EC0: 1.55mg/L
48 hour EC100: 5.49mg/L

Description of key information

Daphnia Magna 48-hour

EC50 - 3.94 mg/L

EC0 - 1.55 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

3.94 mg/L

Additional information

A determination of the acute toxicity to aquatic invertebrates of 2-(Allyloxymethyl) acrylic acid methyl ester was conducted (Mitsubishi Chemical Medience Corporation, 2014, Study A130914).

The study was performed in accordance with OECD test guideline 202, and in compliance with GLP.

Groups of 20 Daphnia magna were exposed to 0 (control group), 1.0, 1.8, 3.2, 5.6, or 10 mg/L 2-(Allyloxymethyl) acrylic acid methyl ester in Elendt M4 Culture medium.

After 24 hours' exposure, 80% Daphnia in the 10 mg/L group were immobilised but no Daphnia in other groups were immobilised. After 48 hours, all of the Daphnia in the 5.6 and 10 mg/L group and 5% of the Daphnia in the 3.2 mg/L group were immobilised, however any of the Daphnia in the lower concentration groups were immobilised.

It was concluded that the 48-hour EC50 in Daphnia Magna was 3.94 mg/L and EC0 in Daphnia Magna was 1.55 mg/L