Registration Dossier

Administrative data

Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: Plant drainage testing method, acute toxicity test of fish and shellfish (71. of JIS K 0102-1998).
Qualifier:
according to
Guideline:
OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Purity: 100% as a reaction product

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
Dispersant: HCO-40
The provided samples and HCO-40 at 20 times its amount were dissolved in acetone, and after distilling the acetone, ion-exchange water was added, so that a stock solution with a test substance concentration of 1,000 mg/L was prepared. The operation was carried out under a light-shielded condition.

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
Kind of fish: Rice fish, Medaka, Oryzias latipes
Reason for selection: Since the sensitivity is similar to that of carp and it is easily obtained as a fish for testing.

Supply source: Ogawa Shoten

Cumulative culture condition: Upon receipt, abnormal fish were removed, and the remaining fish were treated with a medication in a cumulative culture tank and held for 12 days in a flow-water state.

Domestication condition: After the cumulative culture, the fish were transferred to a domestication tank, treated with a medication, and domesticated. During the domestication, abnormal fish were removed, and the remaining fish were held for 15 days in a flow-water state at a water temperature of 25 ± 2ºC. Then, the fish were sorted, treated with a drug, and held for 38 days under flow-through conditions.

Weight: Average 0.22 g
Total length: Average 3.0 cm

Sensitivity test: The 48-h LC50 value of a reference material PCP-Na (pentachlorophenol sodium reagent using the test fish of the same lot was 0.420 mg/L.

Study design

Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Test temperature:
24.1ºC
pH:
7.9
Dissolved oxygen:
At time of exposure start 7.9 mg/L
Before water exchange 6.5 mg/L
Details on test conditions:
Number of provided fish: 10 fish/concentration level
Exposure period: 96 h
Exposure method: Half water cut-off type (water exchange every 8-16 h) -- static renewal
Illumination: Under a light-shielded condition for the exposure period.
Reference substance (positive control):
yes
Remarks:
Pentachlorophenol sodium reagent

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 250 mg/L
Details on results:
96-h LC50 value of the test substance > 250 mg/L

96-h LC50 value was calculated by the Doudoroff method.
The concentration of the dispersant (HCO-40) used at that time was 5,000 mg/L and the 96-h LC50 value of the dispersant was 50,000 mg/L, the test at a concentration higher than that was not carried out in consideration of the influence of the toxicity of the dispersant.

Applicant's summary and conclusion

Conclusions:
96-h LC50 was > 250 mg/L
Executive summary:

This OECD 210 study with Oryzias latipes was part of a BCF study with Carp. The 96-h LC50 was determined to be > 250 mg/L.