3-acetylthiazolidine-4-carboxylic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Deviations from the Study plan (if any) were acknowledged and assessed by the Study Di-rector and included in the Final report.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV -determination.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Species：Danio rerio HAMILTON-BUCHANAN
Age：sexually immature young fish of the same age, length 1 – 2 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

The fish were exposed to the test item for a period of 96 hours. Mortalities and abnormal behaviour were recorded after 1 ± 0.5 h, 4 ± 0.5 h and 24 ± 1 h. On day 2 – 3, mortalities and abnormal behaviour were recorded twice per day (morning and afternoon)

Hardness:

Blank control
Hardness in mg CaCO3/L
Day 0 53.4

Test temperature:

Temperature: 22.5 – 24.3 °C

pH:

7.5 - 7.7

Dissolved oxygen:

O2 values (in % saturation)
Nom. Conc. in mg/L O2 in % at t in h
0 h 24 h 48 h 72 h 96 h
Blank control 100 96.0 95.2 94.7 96.2
100 100 94.3 93.2 94.1 94.9

values (in mg/L)
Nominal Conc. in mg/L O2 in mg/L at t in h
0 h 24 h 48 h 72 h 96 h
Blank control 8.7 8.2 8.0 7.9 7.9
100 8.8 8.0 7.8 7.8 7.8

Salinity:

Nitrate
Blank control
mg NO3/L
Day 0 1

Conductivity:

TOC
Day 0 0.5mg/L

Nominal and measured concentrations:

Date: 20. – 24. Jun. 2022
Treatment: 100 mg/L nominal concentration
Test Design: static
Medium renewal: none
Duration: 96 hours
Loading: 1 fish/L
Aeration: accomplished with glass tubes
Feeding: none
Photo period: 12/12 hours using LED’s
Light intensity: 655 lux
Temperature: 22.5 – 24.3 °C
within one test, temperature did not vary more than 2 °C
between test vessels and between successive days
Test vessels Glass aquaria total volume 12 L
Replicate (Treatment): 1 vessel, each containing 7 L test solution and 7 fish
Replicate (Blank Control): 1 vessel, containing 7 L test medium and 7 fish

Details on test conditions:

Individual size (wet weight and total length) was measured in a subsample of 10 fish from the same batch used in the study, in the week before the start of the test.
At least 24 hours before the start of the test, the test fish were no longer fed.
Before the fish were set into the aquaria, temperature adaptation has taken place.
Temperature, pH and O2-concentrations were recorded every 24 ± 1 hours. Hardness, Ni-trate and TOC were measured at the beginning of the test in the Blank control.
Observations were made after 1 ± 0.5 h, 4 ± 1 h and 24 ± 1 h after the start of the expo-sure (day 0-1). On days 2-3 of the test, all vessels were inspected twice per day (morning and afternoon). On day 4 of the test, all vessels were inspected only once per day in the morning because the test was already finished.
Test criterion is the death of the fish.
The content of the test item in the test vessels was measured at the beginning and at the end of the test.

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Validity criteria fulfilled:

yes

Conclusions:

The following result for the test item 3-acetylthiazolidine-4-carboxylic acid could be deter-mined:
96h-LC50 > 100 mg/L

Executive summary:

One valid experiment was performed.
The toxicity towards Danio rerio was tested using a static test design.
The study was performed as a limit test at the concentration 100 mg/L nominal.
The tested concentration showed no mortality. No fish died or showed any signs of abnor-mal behavior in the blank control and the treatment.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV -determination. The concentration determined at the start of the test was 104 % of the nominal concentration. At the end of the test the determined concentration was 106 % of the nominal concentration. Therefore, the determination of the biological result was based on the nominal concentration (see Guidance Doc. No.23 (§176)).

The following result for the test item 3-acetylthiazolidine-4-carboxylic acid could be deter-mined:

96h-LC50 > 100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L