3-acetylthiazolidine-4-carboxylic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

For the calibration, a dilution series in acetonitrile/water 50/50 (% v/v) was prepared, using a stock solution. The method was validated for the parameters specificity, linearity, instru-ment repeatability, carryover, accuracy and precision. Stability of the test item was tested in a stock solution (1000 mg/L in demineralised water), standard solution samples with the concentration 50 mg/L in acetonitrile/water 50/50 (% v/v) and under test conditions for Daphnia immobilization test.

Test organisms (species):

Daphnia magna

Details on test organisms:

Genus Daphnia
Species magna
Strain STRAUS / BERLIN
Taxonomic position Arthropoda - Crustacea
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin

Test type:

static

Water media type:

other: demineralised water

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Using a glass tube, the daphnia were caught and lifted from the beaker. They were put on a small sieve, and the medium surrounding the animals was sucked off using absorbent pa-per. Immediately after that, the animals were put into the respective test solution.
The test vessels were left to stand for 48 hours. After 24 and 48 hours, the immobilised daphnia were counted. Daphnia are considered immobilised when they do not perform any movements or are only able to move their antennae when the beaker is gently agitated. Daphnia which are trapped at the surface of the test solution are also considered immobi-lised.
The pH, the concentration of dissolved oxygen and the content of the test item in the test vessels were measured at the beginning and at the end of the test.
All values were documented in the raw data.

Test temperature:

19.5 – 20.3 °C

Nominal and measured concentrations:

The measured concentration at the beginning of the test was 100 % of the nominal concen-tration and after 48 hours the measured concentration was 106 % of the nominal concen-tration. Therefore, the determination of the biological results was based on the nominal concentration.

Details on test conditions:

Date of performance: 21. – 23. Jun. 2022
Treatment: 100 mg/L nominal concentration
The concentration to be tested was based on the result of a non-GLP pre-test.
Temperature: 19.5 – 20.3 °C
within one test, temperature did not vary more than 2 °C
Photo period: 16/8 hours, using LED’s
Duration: 48 hours
Observation times: 24 and 48 hours
Medium renewal: none
Replicates (Treatment): 4 vessels, each containing 20 ± 5 mL test solution and 5 daphnia
Replicates (Blank control): 4 vessels, each containing 20 ± 5 mL M4 medium and 5 daphnia

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5) was used as positive controls in a current reference study (202204R201). The 48 h-EC50 against 3,5-Dichlorphenol was determined as 2.04 mg/L.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Validity criteria fulfilled:

yes

Conclusions:

The following result was determined for the test item 3-acetylthiazolidine-4-carboxylic acid (species: Daphnia magna):
48h-EC50 > 100 mg/L (nominal)

Executive summary:

One valid experiment was performed.
The study was performed as a limit test at the concentration 100 mg/L (nominal). For the test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
The tested concentration showed no toxicity. One of the animals was immobilised in the blank control which can be stated as not significant.
3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5) was used as positive control in a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV -determination. The concentration determined at the start of the test was 100 % of the nominal concentration. At the end of the test the determined concentration was 106 % of the nominal concentration. Therefore, the determination of the biological result was based on the nominal concentration (see Guidance Doc. No.23 (§176)).

The following result was determined for the test item 3-acetylthiazolidine-4-carboxylic acid (species: Daphnia magna):

48h-EC50 > 100 mg/L (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L