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EC number: 480-890-9 | CAS number: 906532-68-1
Concurrent Cytotoxicity analysis of Everzol Orange ED-G Crude in Chinese Hamster Ovary Cells
Summary of Chromosome aberrations in Chinese Hamster Ovary cells
The chromosome aberration assay was conducted in three test schemes: 3-hour exposure both with and without S9 activation (schemes I and II) and 20-hour continuous exposure without S9 (scheme III). Results were conducted in duplicate cultures and with negative control and positive controls concurrently. 100 metaphases for each culture and 200 metaphases for each treatment were scored. Results showed that percents of aberrant cells induced by negative control in the scheme I, II and III were 1%, 0% and 0.5%. The positive control induced significant increases in percents of aberrant cells over the corresponding negative control. Everzol Orange ED-G did not significantly increase the frequencies of structural chromosome aberration in the schemes I and III. However, the frequencies of structural chromosome aberration in the scheme II, at concentration of 150 and 500 um/mL in the 3 hr incubation have significantly increased.
In conclusion, Everzol Orange ED-G crude induced structural chromosome aberration at the concentration of 150 and 500 μg/mL at the presence of S9 for 3-hr treatment. The concentration-response analysis and trend probability were conducted in the 3-hr treatment with S9 and results showed lacking in concentration response.
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