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Only short-term toxicity studies are available. The analyses of three trophic (fish, invertebrates, algae) levels have not revealed significant toxic effects. At the short-term fish, no mortality is observed at the highest nominal concentration of 100 mg/l. No immobilization of Daphina is observed at the highest nominal concentration of 100 mg/l. The extrapolation EC 50 of algae is more than 100 mg/l.

Activated sludge bacteria showed a low sensitivity to Everzol Orange ED-G Crude. There is no effect at the concentration of 1000 mg/l.

Consequently, it can be concluded that Everzol Orange ED-G Crude has no acute adverse effects on aquatic organisms.