Registration Dossier

Administrative data

Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental starting date: 05 July 2017. Experimental completion date: 23 August 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
flask method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Identification: Beta-Menadione
CAS No: 58-27-5
Description: light yellow powder
Batch number: J16090701
Purity: 99.2%
Expiry date: 06 September 2017
Storage conditions: room temperature, in the dark

Results and discussion

Water solubility
Water solubility:
0.091 g/L
Conc. based on:
test mat.
Temp.:
20 °C
pH:
5.8
Details on results:
Preliminary Test
The preliminary estimate of the water solubility at 20.0 ± 0.5 °C was 0.104 g/L. The solution had a pH of 5.7.

Validation
The linearity of the detector response with respect to concentration was assessed over the nominal concentration range of 10 to 100 mg/L. The results were satisfactory with a correlation coefficient (r) of 0.998 being obtained.
Assessment of the sample recovery procedure was performed and proved adequate for the test; at a nominal concentration of 102 mg/L, a mean percentage recovery of 92.3% was obtained (range 91.6 to 92.8%). Test sample concentrations have been corrected for the recovery of analysis.

Discussion
On completion of the equilibration period, the samples were clear and colorless with excess test item present. After filtration the solutions were visually observed to be clear, pale yellow solutions with a faint Tyndall beam effect. The intensity of the yellow color of the excess test item may be the reason the solutions appeared colorless prior to filtration.
All individual water solubility results were within ± 15% of the mean, thus satisfying the criteria specified in the guidelines.

Any other information on results incl. tables

Definitive Test

The concentration (g/L) of test item in the sample solutions is shown in the following table:

Sample Number Time Shaken at
30 ºC (hours)
Concentration (g/L) Solution pH
1 72 9.22 x 10^-2 5.9
2 48 9.15 x 10^-2 5.7
3 24 9.07 x 10^-2 5.7

Mean concentration: 9.15 x 10-2 g/L at 20.0 ± 0.5 ºC

Applicant's summary and conclusion

Conclusions:
The water solubility of the test item has been determined to be 9.15 x 10-2 g/L of solution at 20.0 ± 0.5 °C.
Executive summary:

Water Solubility. 9.15 x 10-2 g/L of solution at 20.0 ± 0.5 °C, using the flask method, designed to be compatible with Method A.6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.