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In the assessment of magnesium 2-ethylhexanoate, read-across to the assessment entities soluble magnesium substances and 2-ethylhexanoic acid is applied since the ions of magnesium 2-ethylhexanoate determine its fate and toxicity in the environment. Magnesium is essential for microorganisms and is therefore added to the synthetic sewage feed according to the “activated sludge respiration inhibition test (OECD 209, 2010). Effects on activated sludge endogenous respiration were not observed by Liu and Wang (2012) up to the highest magnesium concentration tested (NOEC ≥ 500 mg Mg/L, measured). Regarding 2-ethylhexanoate, an inhibition of bacterial growth was observed yielding a 17-h EC10 of 71.7 mg/L and a 17-h EC50 of 112.1 mg/L 2-ethylhexanoate.

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