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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 - 15 May 1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
other: BIO-D MIC 286-7
GLP compliance:
no
Vehicle:
not specified
Test organisms (species):
activated sludge
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
25 other: % dilution
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
98 g/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition
Remarks on result:
other: 25% dilution of product (39.18% concentration) is equivalent to 98 g/l
Duration:
48 h
Dose descriptor:
other: Minimum Inhibitory Concentration
Effect conc.:
50 other: % dilution
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

After 48 hours, growth was not inhibited at concentrations up to 25%. No growth was observed in one of the two vessels at 50%, whereas in the other vessel no growth inhibition was observed. Thus the MIC was 50%. No inhition was observed at 25% dilution of the product (NOEC).

Validity criteria fulfilled:
not specified
Conclusions:
The test substance (Diacid 1550 Potassium salt (6339-33), 39.18% concentration) did not inhibit the growth of activated sludge up a 25% dilution of the product. This is recalculated to a concentration for the active of 98 g/L.
Executive summary:

The Minimum Inhibitory Concentration (MIC) was tested according to the method BIO-D MIC 286-7.

After 48 hours contact in water inoculated with sewage microbes, the sample was non-inhibitory to sewage organisms at concentrations of 25% or less.

Description of key information

The test substance (Diacid 1550 Potassium salt (6339-33), 39.18% concentration) did not inhibit the growth of activated sludge up a 25% dilution of the product. This is recalculated to a concentration for the active of 98 g/L.

The Minimum Inhibitory Concentration (MIC) was tested according to the method BIO-D MIC 286-7.

After 48 hours contact in water inoculated with sewage microbes, the sample was non-inhibitory to sewage organisms at concentrations of 25% or less.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
98 g/L

Additional information