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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
other: 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
not specified
Remarks:
Only signatures for GLP test plan available, GLP for test itself unclear
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 6.25, 12.5, 25, 50 and 100 mg/l (nominal)
- Sampling method: A two milliliter aliquot of each sample solution was withdrawn for analysis
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: No solvent was required to prepare the test solutions
- Controls: Yes
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Fathead minnow
- Source: Chesapeake cultures
- Age at study initiation (mean and range, SD): 28 - 31 days
- Length at study initiation (length definition, mean, range and SD): <=18 mm
- Feeding during test: No feeding during test

ACCLIMATION
- Type and amount of food: Laboratory prepared diet of commercial food (Zing/Tetramin)
- Feeding frequency: twice a day
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not relevant
Hardness:
70 - 88 mg/l CaCO3
Test temperature:
22 °C
pH:
7.1 - 8.0
Dissolved oxygen:
4.5 - 8.2
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal: 6.25, 12.5, 25, 50 and 100 mg/l
Measured: 2.4, 4.9, 9.8, 16.3 and 41.3 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: vessel
- Material, size, headspace, fill volume: polypropylene, 2L filled with 1L test medium
- Aeration: Aerate by mixing test solutions to saturation prior to test; if dissolved oxygen falls below 40 percent saturation in any replicate during the test, supply oil free air at 100 ±10 bubbles per minute.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: US EPA moderately-hard reconstituted water:
NaHCO3 - 96 mg/L
CaS04.2H20 - 60 mg/L
MgSO4 - 60 mg/L
KCl - 4 mg/L

- Alkalinity: 72 - 100 mg/L CaCO3
- Conductivity: 232 - 248 umhos
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, pH, oxygen and conductivity were recorded daily

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: 50 to 100 ft candles

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, reflex loss, erratic swim are recorded daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
18.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
17 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
16 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
15 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
9.8 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Details on results:
- Mortality of control: 0%
Results with reference substance (positive control):
Not relevant
Reported statistics and error estimates:
Probit Analysis or Spearman - Karber Method when possible, otherwise, graphical interpolation

For detailed results, see attached file "Results.doc".

Validity criteria fulfilled:
yes
Remarks:
Mortality in controls at 0%, oygen saturation above 60%
Conclusions:
The acute toxicity (96h-LC50) of Diacid 1550 potassium salt towards Pimephales promelas is 15 mg/L based on the active ingredient.
Executive summary:

The acute toxicity of Diacid 1550 potassium salt towards Pimephales promelas was investigated according to 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7 under GLP. Fish were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/l and were observed for 96 hours. Based on measured concentrations, the 48h- and 96h-LC50 and 96h-NOEC were found to be 17, 15 and 9.8 mg/L respectively based on the active ingredient.

Description of key information

The acute toxicity (96h-LC50) of Diacid 1550 potassium salt towards Pimephales promelas is 15 mg/L based on the active ingredient.

Key value for chemical safety assessment

LC50 for freshwater fish:
15 mg/L

Additional information

The acute toxicity of Diacid 1550 potassium salt towards Pimephales promelas was investigated according to 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7 under GLP. Fish were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/L and were observed for 96 hours. Based on measured concentrations, the 48h- and 96h-LC50 and 96h-NOEC were found to be 17, 15 and 9.8 mg/L respectively based on the active ingredient.