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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable and sufficient documented

Data source

Reference
Reference Type:
publication
Title:
Fetal toxicity of zinc ethylphenylditioncarbamate (ZEPC) (3.). Mechanism of formation of methemoglobin caused by ZEPC
Author:
Nakaura S, Kawanishi T, Ohno Y, Kawashima K, Tanaka S, Takahashi S, Takanaka A, Omori Y, Matumoto K
Year:
1985
Bibliographic source:
J Toxicol Sci 10, 252

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
After oral administration of 1000 mg/kg of ZEPC to Wistar rats female rats, the metabolites in the blood were extracted with ethyl acetate.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Zinc bis(N-ethyl-N-phenyldithiocarbamate)
EC Number:
238-677-1
EC Name:
Zinc bis(N-ethyl-N-phenyldithiocarbamate)
Cas Number:
14634-93-6
Molecular formula:
C18H20N2S4Zn
IUPAC Name:
zinc bis(N-ethyl-N-phenyldithiocarbamate)
Specific details on test material used for the study:
No data.
Radiolabelling:
no

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
No data

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Details on exposure:
No data
Duration and frequency of treatment / exposure:
No data
Doses / concentrations
Dose / conc.:
1 000 mg/kg bw/day (nominal)
No. of animals per sex per dose / concentration:
No data
Control animals:
not specified
Positive control reference chemical:
No data
Details on study design:
Metabolites in the blood were extracted with ethyl acetate and identified by GC-MS.

Results and discussion

Main ADME results
Type:
metabolism
Results:
N-ethylaniline and Aniline were identified as metabolites

Toxicokinetic / pharmacokinetic studies

Details on absorption:
No data
Details on distribution in tissues:
No data
Details on excretion:
No data

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
N-ethylaniline and Aniline were identified as metabolites.

Applicant's summary and conclusion

Conclusions:
N-ethylaniline and Aniline were idetified as metabolites.
Executive summary:

After oral adminisatration of 1000 mg/kg of ZEPC to Wistar rats female rats, the metabolites in the blood were extracted with ethyl acetate. N-ethylaniline (N-EA) and Aniline (AN) were identified as metabolites. N-EA and AN concentrations in the blood reached maximum levels (10.0 and 2.01 µg/ml) after 24 hr and then decreased to less than 0.3 µg/ml within 3 days. The increased methemoglobin levels in the blood after administration of ZEPC were nearly proportional to the changes in blood concentrations.