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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2005-01 to 2005-02
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP-Guideline study. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes (incl. certificate)
Remarks:
Landesamt für Umweltschutz und Gewerbeaufsicht, Rheinland-Pfalz, Germany

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Ca++-loaded Zeolite X/A (80/20%)
- Chemical name: Zeolite, cuboidal, crystalline, synthetic, non-fibrous
- Framework: cuboidal
- Related CAS number: 1318-02-1
- Analytical purity: 100%
- Lot/batch No.: 9323
- Expiration date of the lot/batch: 31.12.2006
- Chemical name: Ca-Aluminosilicate
- Storage condition of test material: room temperature
- surface modified with Ca

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
Since the test substance is poorly water soluble, a saturated solution was prepared by shaking a nominal load of 1000 mg/L with deionized water for 24 hours, followed by membrane filtration.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Newborn daphnia (strain: STRAUS, aged 0 to 23 hours, bred in the LAUS GmbH according to EN ISO 6341, originally obtained from Federal Environment Agency Berlin) were transferred to M4 Elendt medium for a settling-in period of 30 minutes.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Hardness:
157-283 mg CaCO3/L
Test temperature:
19.7 to 22.4°C
pH:
7.7 to 8.5
Dissolved oxygen:
7.7 to 9.4 mg/L
Nominal and measured concentrations:
0, 10, 32, 100, 320 and 1000 mg/L (nominal)
Details on test conditions:
Newborn daphnia (aged 0 to 23 hours, bred in the LAUS GmbH according to EN ISO 6341, originally obtained from Federal Environment Agency Berlin) were transferred to M4 Elendt medium for a settling-in period of 30 minutes. Undamaged individuals were caught using a glass tube (diameter 8 mm) and put into the respective test vessel (glass beakers, 100 mL) containing the test solution (loading 80 mL/animal, animals were held individually, 10 replicates/treatment, no aeration).
Following nominal concentrations were tested: 0, 10, 32, 100, 320 and 1000 mg/L (dilution water: tap water, TOC 0.82 mg/L, pH 8.17, conductivity 249 µS/cm, hardness 0.943 mmol/L; enriched to M4 Elendt medium).
Test vessels were inspected for offspring daily. Newborn daphnids were counted and removed from the test vessel.
Medium renewal was 3 x per week using freshly prepared test solutions.
pH and oxygen were measured in both the old and the new test solutions, hardness was determined once a week.
Daphnids were fed with Desmodesmus subspicatus (0.15-0.20 mg org. C/animal/day).
Statistical significance was tested by means of t-test or Weir-test, if homogeneity of variances was not given. EC50 was calculated according to a sigmoidal fit of the data (OriginTM).
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
eluate
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
eluate
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
680 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
eluate
Basis for effect:
reproduction
Details on results:
Temperature during the test was 19.7 to 22.4°C, pH was 7.7 to 8.5, hardness was 157-283 mg CaCO3/L and O2 content was 7.7 to 9.4 mg/L. Lighting was below 2000 lux.
The NOEC was 32 mg/L, the corresponding LOEC 100 mg/L and the EC50 680 mg/L, based on nominal concentrations.
Mortality in the control was 0%, mean offspring was 96.
Following mean offspring numbers (in brackets inhibition of reproduction compared to the control) were obtained for the tested concentrations: 10 mg/L 104 (-9%), 32 mg/L 100 (-5%), 100 mg/L 74 (22%), 320 mg/L 82 (14%) and 1000 mg/L 30 (69%).
Mortality was 10% in the 320 mg/L treatment and 40% in the 1000 mg/L treatment. Results for the treatments 100 and 1000 mg/L were statistically significant (p<0.01).

Any other information on results incl. tables

Summary of results:

Treatment (mg/L)

Total offspring

Surviving parent animals

Offspring/ surviving parent animal

Inhibition of reproduction (%)

control

958

10

96

0

10

1043

10

104

-9

32

1003

10

100

-5

100

743

10

74

22

320

739

9

82

14

1000

177

6

30

69

Applicant's summary and conclusion