reaction mass of (9Z,11E)-octadeca-9,11-dienoic acid and (10E,12Z)-octadeca-10,12-dienoic acid and octadec-9-enoic acid

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Oleic acid

EC Number:

204-007-1

EC Name:

Oleic acid

Cas Number:

112-80-1

Molecular formula:

C18H34O2

IUPAC Name:

octadec-9-enoic acid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was added directly to the test media a) without additional ultracentrifugation at 10000 mg/L product; b) with additional ultracentrifugation (15 min) at 3000 mg/L product.

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

Species: Pseudomonas putida, Strain Berlin 33/2 (DSM 50026)
Origin: Stammsammling TFB-Mirkobiologie, Henkel KGaA

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test conditions

Hardness:

not specified

Test temperature:

room temperature (not specified)

pH:

not specified

Dissolved oxygen:

> 2 mg/L

Nominal and measured concentrations:

Nominal: 0, 3000, 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vials
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 ml fill volume
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to DIN 38412 - part 27

OTHER TEST CONDITIONS
- Adjustment of pH: not indicated
- Photoperiod: not relevant
- Light intensity: not indicated


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption at 3 h

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 3 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Reported statistics and error estimates:

The calculation of the oxygen consumption and the mean values was carried out using a LOTUS calculation program.