reaction mass of (9Z,11E)-octadeca-9,11-dienoic acid and (10E,12Z)-octadeca-10,12-dienoic acid and octadec-9-enoic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

test duration was only 48 h instead of 96 h

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Stearic acid

EC Number:

200-313-4

EC Name:

Stearic acid

Cas Number:

57-11-4

Molecular formula:

C18H36O2

IUPAC Name:

stearic acid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Preparation of the test solution:
Direct weight of the test substance into the test vessels, filled up to final volumes of 10 l with copper- and chlorine-free drinking water, then stairing 5 mitmtes by ultraturrax.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden Orfe
- Source: not indicated
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): about 6 cm


ACCLIMATION
- Acclimation period: according to OECD 203
- Acclimation conditions (same as test or not):according to OECD 203
- Type and amount of food during acclimation: according to OECD 203
- Feeding frequency during acclimation:according to OECD 203
- Health during acclimation (any mortality observed): not indicated

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

about 16 °dH ( about 93 mg Ca and 12 mg Mg per litre)

pH:

7.6.-8.4

Dissolved oxygen:

76 - 94 % O2 saturation

Nominal and measured concentrations:

Nominal: 1000, 3000, 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L basins
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 10 L
- Aeration: not indicated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 203
- Intervals of water quality measurement: not indicated

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16: 8 h day night regime
- Light intensity: not indicated

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms of intoxication

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx 3

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Additional remarks: The fish did not show any abnormal behaviour.

Reported statistics and error estimates:

Not applicable, due to the absence of any toxic effect uo to the highest concentration tested.