reaction mass of (9Z,11E)-octadeca-9,11-dienoic acid and (10E,12Z)-octadeca-10,12-dienoic acid and octadec-9-enoic acid

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2015

Materials and methods

Test guideline

Qualifier:

no guideline followed

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

Test material form:

liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: inhouse breeding
- Housing: individually after confirmation of pregnancy
- Age at study initiation: adult, 110 days
- Diet: standard purified control diet (CD), standard purified control diet with c9,t11-CLA (CLA), high fat diet (HF), HF with c9,t11-CLA (HFCLA)

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: feed

Vehicle:

unchanged (no vehicle)

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

P0 females: 160 days
F1: 150 days

Frequency of treatment:

Daily

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

0 other: %

Remarks:

control diet (CD)

Doses / concentrations 2

Dose / conc.:

0 other: %

Remarks:

high fat diet (HF)

Doses / concentrations 3

Dose / conc.:

1 other: %

Remarks:

control diet with c9,t11-CLA (CLA)

Doses / concentrations 4

Dose / conc.:

1 other: %

Remarks:

HF with c9,t11-CLA (HFCLA)

No. of animals per sex per dose:

7 dams
8 pups/litter

Control animals:

yes, plain diet

Details on study design:

Female rats were fed for 10 days prior to time mated. Mating point was determined using a estrous cycle monitor (EC-40; Fine Science Tools).

Statistics:

Statistical analysis was performed using SigmaPlot 12.0 (Systat Software Inc.). All data were analyzed by two-way factorial ANOVA, with maternal HF and maternal CLA intake as factors. Holm-Sidak post hoc tests were performed where indicated to detect further differences between groups.

Results and discussion

Effect levels

Key result

Sex:

male/female

Remarks on result:

other: no treatment related adverse effects were observed.

Observed effects

The age of pubertal onset was significantly reduced in high fat diet (HF) compared to control diet (CD), control diet with conjugated linoleic acid (CLA), and high fat diet with conjugated linoleic acid (HFCLA) offspring. The weight at puberty was significantly increased in HF offsprings.
HF offspring had significantly increased plasma leptin concentrations compared to CD, CLA, and HFCLA offspring. Triglyceride as well as cholesterol concentrations were significantly increased in HF compared to CD, CLA, and HFCLA fed animals. An significant increase in hepatic IGF-1 expression in HF-offsprings at P24 was observed compared to all other groups, but no significant differences were observed at P150. IL-1b and IL-6 expression was increased in HF-offsprings compared to all other groups. However, there was no difference between groups in TNFa or IL-10 expression. CD36, DGAT1, and FASN were significantly upregulated in offspring of HF mothers compared with CD, CLA, and HFCLA offspring. ApoE was significantly increased in HF and HFCLA compared to CD and CLA groups. Female HF-offsprings showed prolonged or irregular estrous cycles compared to CD, CLA, and HFCLA groups.