Registration Dossier
Registration Dossier
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EC number: 274-092-8 | CAS number: 69709-01-9
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
A 72 hour EC50 value of >131 mg/l and NOEC value of >=131 mg/l (geometric mean) (highest concentration tested) have been determined for the effects of the hydrolytic degradation product of the test item 1,1,3,3 -tetramethyl-2-[3-(trimethoxysilyl)propyl]guanidine, on growth rate of Pseudokirchneriella subcapitata.
Key value for chemical safety assessment
Additional information
A 72 hour EC50 value of >131 mg/l and NOEC value of >=131 mg/l (measured, geometric mean) (highest concentration tested) have been determined for the effects of the hydrolytic degradation product of the test item 1,1,3,3 -tetramethyl-2-[3-(trimethoxysilyl)propyl]guanidine, on growth rate of Pseudokirchneriella subcapitata, in accordance with OECD guideline 201. No inhibition of growth was found after 72 hours.
pH adjusted test solutions were included in the study since test item can hydrolyse rapidly at higher pH values. However, as the substance is such a strong base (guanidine) it was not possible to adjust the pH enough to prevent rapid hydrolysis. The concentration level 100 mg/L was tested pH-adjusted (pH 8.2 ± 0.5) and un-adjusted to also distinguish toxic effects of the test item from pH effects.
In view of the test media preparation method and exposure regime it is likely that the test organisms were exposed predominantly to the hydrolysis products of the tested substance. The study was supported by LC-MS/MS analysis of the silanol hydrolysis product in fresh and aged media. The measured concentrations of the hydrolysis product in fresh media at the start of the exposure and aged media at the end of exposure (0 and 72 hours) were in the range of 118 to 132% of the nominal values.
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