Registration Dossier

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Biodegradation:

Biodegradation in water:

Biodegradability of test chemical Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (Direct Blue) CAS no.28407-37-6 was predicted by using OECD QSAR tool box v 3.3 by considering eight closest read across chemicals with log Kow as primary descriptor. Percent biodegradability of test chemical  Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (Direct Blue) was estimated to be 0.625% by considering BOD as parameter and microorganisms as inoculum in 28 days. On the basis of percent biodegradability value it can be concluded that  test chemical can be considered as not readily biodegradable.

Bioaccumulation:

Bioaccumulation: aquatic/sediments:

Based on Annex IX, Column 2 the study does not need to be conducted because the substance tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) has low potential for bioaccumulation based on log Kow<= 3. The log Kow of target chemical tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) is -0.77 dimensionless.

Transport and distribution:

Adsorption/desorption:

Based on Annex VIII, Column 2 the study does not need to be conducted because the substance tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) has low octanol water partition coefficient. The log Kow of target chemical tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) is -0.77 dimensionless and adsorption potential of this substance is related to this parameter.

Additional information

Biodegradation:

Biodegradation in water:

Predicted data studies for target chemical Copper[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (Direct Blue) CAS no.28407 -37 -6 and experimental studies for its structurally similar read across chemical have been conducted for biodegradation in water endpoint and their results are summarized below.

 

In first predicted data study the biodegradability of test chemical Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (Direct Blue) CAS no.28407 -37 -6 was predicted by using OECD QSAR tool box v 3.3 by considering eight closest read across chemicals with log Kow as primary descriptor. Percent biodegradability of test chemical  Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (Direct Blue) was estimated to be 0.625% by considering BOD as parameter and microorganisms as inoculum in 28 days. On the basis of percent biodegradability value it can be concluded that test chemical can be considered as not readily biodegradable.

 

Another predicted data study was done by using Estimation Programs Interface Suite (EPI suite, 2017) in this study the EPI suite was run to predict the biodegradation potential of the test compound Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (CAS no. 28407-37-6) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical  Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) is expected to be not readily biodegradable.

Next study was experimental study and it is done from journal Chemosphere, Vo1.15, No.4, pp 479-491, (1986) by U. Pagga and O. Brown for structurally similar read across chemical chemical tetrasodium 3,3'-[(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4,5-dihydroxynaphthalene-2,7-disulphonate](CAS no.4198 -19 -0) in this study the aerobic biodegradation experiment was performed for chemical tetrasodium 3,3'-[(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4,5-dihydroxynaphthalene-2,7-disulphonate] using activated sludge at concentration 0.5 g/L dry material as inoculums and initial concentration of chemical taken was 100mg/L for 42 days. By considering DOC removal parameter test chemical showed -17 % degradation in 42 days. The read across chemical tetrasodium 3,3'-[(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4,5-dihydroxynaphthalene-2,7-disulphonate] belongs to D category according to table 1 and 2 as its limit value falls in that range. This percentage value is in negative So it is concluded that Tetrasodium 3,3'-[(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4,5-dihydroxynaphthalene-2,7-disulphonate](CAS no. 4198 -19 -0) is not readily biodegradable.

 

Similarly one more experimental study was also done from same source as mentioned above for structurally similar read across chemical tetrasodium;3-[[4-[[4-[(2E)-2-(6-amino-1-oxo-3-sulfonatonaphthalen-2-ylidene)hydrazinyl]-6-sulfonatonaphthalen-1-yl]diazenyl]naphthalen-1-yl]diazenyl]naphthalene-1,5-disulfonate (CAS no. 4399 -55 -7) in this study the aerobic biodegradation experiment was performed for read across chemical tetrasodium;3-[[4-[[4-[(2E)-2-(6-amino-1-oxo-3-sulfonatonaphthalen-2-ylidene)hydrazinyl]-6-sulfonatonaphthalen-1-yl]diazenyl]naphthalen-1-yl]diazenyl]naphthalene-1,5-disulfonate using activated sludge at concentration 0.5 g/L dry material as inoculums and initial concentration of chemical taken was 100 mg/L for 42 days. By considering DOC removal parameter test chemical showed -27 % degradation in 42 days. This percentage value is in negative So it is concluded that read across chemical tetrasodium;3-[[4-[[4-[(2E)-2-(6-amino-1-oxo-3-sulfonatonaphthalen-2-ylidene)hydrazinyl]-6-sulfonatonaphthalen-1-yl]diazenyl]naphthalen-1-yl]diazenyl]naphthalene-1,5-disulfonate is not readily biodegradable.

 

Another experimental study was done from authoritative database (J check, 2017) in this study Biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of read across substance disodium 4 -hydroxy-7 -{[(5 -hydroxy-7 -sulfonato-2 -naphthyl)carbamoyl]amino}naphthalene-2 -sulfonate (CAS no. 20324 -87 -2). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of read across substance disodium 4 -hydroxy-7 -{[(5 -hydroxy-7 -sulfonato-2 -naphthyl)carbamoyl]amino}naphthalene-2 -sulfonate was determined to be 0 and 1.3 % by BOD and TOC removal parameter respectively in 14 days. Thus, based on percentage degradation, disodium 4 -hydroxy-7 -{[(5 -hydroxy-7 -sulfonato-2 -naphthyl)carbamoyl]amino}naphthalene-2 -sulfonate is considered to be not readily biodegradable in nature.

 

By considering results of predicted data for target chemical and experimental studies of structurally similar read across chemicals it can be concluded that test chemical Copper,[tetrahydrogen-3,3'-[(3,3'-dihydroxy-4,4'-biphenylylene)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonato](4-)]di-,tetrasodium salt (7CI) (Direct Blue) CAS no.28407 -37 -6 is not readily biodegradable.

Bioaccumulation:

Bioaccumulation: aquatic/sediments:

Based on Annex IX, Column 2 the study does not need to be conducted because the substance tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) has low potential for bioaccumulation based on log Kow<= 3. The log Kow of target chemical tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) is -0.77 dimensionless.

Transport and distribution:

Adsorption/desorption:

Based on Annex VIII, Column 2 the study does not need to be conducted because the substance tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) has low octanol water partition coefficient. The log Kow of target chemical tetrasodium (3E)-5-amino-3-{2-[4-(4-{2-[(2E)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3- hydroxyphenyl)-2-hydroxyphenyl]hydrazin-1-ylidene}-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate dicopper (CAS no. 28407-37-6) is -0.77 dimensionless and adsorption potential of this substance is related to this parameter.