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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journal
Reference:
Composition 0
Composition 0
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To evaluate the acute toxicity of Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)) on Fathead Minnow fish.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material : C.I. Direct Blue 218
- Molecular formula : C32H20Cu2N6Na4O16S4
- Molecular weight : 1087.82 g/mol
- Smiles notation : c12c3c(c(S(=O)(=O)[O-])cc1cc(S(=O)(=O)[O-])cc2N)N=Nc1ccc(cc1O[Cu]O3)c1cc2c(N=Nc3c(cc4c(c3O[Cu]O2)c(cc(c4)S(=O)(=O)[O-])N)S(=O)(=O)[O-])cc1.[Na+].[Na+].[Na+].[Na+]
- InChl : 1S/C32H24N6O16S4.2Cu.4Na/c33-19-11-17(55(43,44)45)5-15-9-25(57(49,50)51)29(31(41)27(15)19)37-35-21-3-1-13(7-23(21)39)14-2-4-22(24(40)8-14)36-38-30-26(58(52,53)54)10-16-6-18(56(46,47)48)12-20(34)28(16)32(30)42;;;;;;/h1-12,39-42H,33-34H2,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;;;/q;2*+2;4*+1/p-8/b37-35-,38-36-;;;;;;
- Substance type: Organic
- Physical state: solid powder
Analytical monitoring:
not specified
Details on sampling:
composite stored a t 20 C in dark
Stock solution refrigerated
Vehicle:
not specified
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Fathead Minnow
- Source: Berry water gardens , windmill fish hatcheries , North Carolina
- Length at study initiation : 5.2 cm
- Weight at study initiation: 1.3 g
- Feeding during test: starved 48 hrs before test

ACCLIMATION
- Acclimation period: 10 days
- Acclimation conditions : maintained in plastic stock tank in continuously running charcoal –sand filtered Chapel Hill tap water
- Type and amount of food: protein enriched fish food
- Feeding frequency: 5-7 times per week
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
Observations were made after every 24 h
Hardness:
32mg/l
Test temperature:
17°C
pH:
6.8-8.5
Nominal and measured concentrations:
0,18,32,56,100,180 mg/l
Details on test conditions:
Test vessel: 5-gallon wide mouth glass jar
- No. of organisms per vessel: 10
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: No other data

Dye concentration mg/l

No. test fish

No. of fish surviving

 

 

 

24h

48h

72h

96h

0

10

10

10

10

9

18

10

10

10

10

10

32

10

10

10

10

9

56

10

10

10

10

10

100

10

10

10

10

9

180

10

10

10

10

10

Validity criteria fulfilled:
not specified
Conclusions:
Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) when subjected to Fathead Minnow (Pimephales promelas) for 96 hrs produced no toxicity and the observed effect concentration was > 180 mg/l.
Executive summary:

Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

  10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l

By considering LC50 value it is concluded that test chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria

Description of key information

Short-term toxicity to fish:

Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

  10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l

By considering LC50 value it is concluded that test chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria

Key value for chemical safety assessment

LC50 for freshwater fish:
180 mg/L

Additional information

Short-term toxicity to fish:

Experimental study for short-term toxicity to fish was summarized in Handbook of Environment and Waste Management air and water pollution control , Yung-Tse Hung , Lawrence K Wang, Nazih K Shammas 2012 and Ecology committee American Dye Manufacturers Institute, INC. Sept. 1972 for target chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)) (CAS 28407-37-6).

Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

  10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l

By considering LC50 value it is concluded that test chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria