Diallyl 2,2'-oxydiethyl dicarbonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982-10-18 to 1982-10-22

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

Guideline study without GLP and analytics but acceptable for assessment.

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPP 72-1 (Fish Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

no

Remarks:

only raw data were stored

Analytical monitoring:

no

Remarks:

But due to good water solubility and slow hydrolysis the test item is expected to be stable for the duration of the test.

Details on sampling:

- Concentrations: 0.22, 0.36, 0.6, 1.0 and 1.7 mg/L (nominal concentrations)
- Sampling method: No analytical verification of nominal test substance concentrations

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: Untreated control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): No vehicle used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Not relevant

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Common name: Bluegill
- Strain: Information not available
- Source: Fish were obtained from a commercial fish supplier in Missouri
- Age at study initiation (mean and range, SD): Information not available
- Length at study initiation (length definition, mean, range and SD): Mean (range, N=30) total length of 29 (24-35) millimeters.
- Weight at study initiation (mean and range, SD): Mean (range, N=30) wet weight of 0.26 (0.14-0.45) grams.
- Feeding during test
- Food type: No feeding during test
- Amount: No feeding during test
- Frequency: No feeding during test


ACCLIMATION
- Acclimation period: Test fish were maintained under these conditions for a minimum of 14 days.
- Acclimation conditions (same as test or not): 500-L fiberglass tank
- Type and amount of food: All fish were fed a dry pelleted food, ad libitum, daily except during the 48 hours prior to testing.
- Feeding frequency: Ad libitum
- Health during acclimation (any mortality observed) : There was 0.2% mortality in the test fish population during 2 day period prior to test initiation

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

hardness and alkalinity ranges as calcium carbonate (CaC03) of 22-26 mg/L and 16-24 mg/L, respectively

Test temperature:

The temperature in the holding tank was 22-21°C:
At 0 hours: 22°C,
At 24 h: 22°C,
At 48 h: 22°C,
At 72 h: 22°C,
At 96 h: 21°C.

pH:

At 0 hours: 7.8,
At 24 h: 7.5 to 7.6,
At 48 h: 7.3 to 7.4,
At 96 h: 6.9 to 7.1.

Dissolved oxygen:

DO (mg/l):
At 0 hours: 9.2 to 9.5,
At 24 h: 8.1 to 8.3,
At 48 h: 7.3 to 7.7
At 96 h: 2.5 to 6.6

Salinity:

Specific conductance range of 90-110 µmhos/cm

Nominal and measured concentrations:

0.22, 0.36, 0.6, 1.0 and 1.7 mg/L (nominal concentrations);
no analytical verification of nominal test substance concentrations, but due to good water solubility and slow hydrlysis the test item is expected to be stable for the duration of the test.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: The definitive test was conducted in 19.6 L glass jars which contained 15 L of test solution.
- Aeration: no information available
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: no information available
- Total organic carbon: no information available
- Alkalinity: This water had a total hardness and alkalinity as CaC03 of 46 mg/L and 30 mg/L, respectively.
- Ca/mg ratio : no information available
- Conductivity: 150 µmohs/cm
- Intervals of water quality measurement: The pH's and DO concentrations were measured at 0, 24, 48 and 96 hours in the control and the high,
middle and low test concentrations.


OTHER TEST CONDITIONS
- Adjustment of pH: pH 7.8
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: not reported
The temperature in the holding tank was 22-23°C

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortalities were recorded and removed from each test jar and biological observations of the fish and observations of the physical characteristics of
the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours of exposure.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.57 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: estimated by moving average angle analysis; 95% CL = 0.45-0.73

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

0.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: estimated by moving average angle analysis; 95% CL = 0.47-0.76

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.77 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: estimated by binomial probability; 95% CL = 0.60-1.0

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: estimated by binomial probability; 95% CL = 0.60-1.7

Details on results:

The concentrations tested and the corresponding mortality data derived from the toxicity test were used to estimate 24-, 48-, 72- and 96-hour median lethal concentrations (LC50) and 95% confidence intervals. The 96-hour LC50 (and 95% confidence interval) for bluegill exposed to CR-39, estimated by moving average angle analysis, was 0.57 (0.45-0.73) mg/L.

Results with reference substance (positive control):

No reference substance investigated in this test.

Reported statistics and error estimates:

The computer program utilized (Stephan 1978, personal communication) estimated LC50 values using one of three statistical methods in the following order of preference: moving average angle analysis, probit analysis, binomial probability. The method selected was determined by the characteristics of the data base (i.e. presence or absence of test concentrations causing mortality of 100% of the animals in the test population, test concentrations causing mortality of a partial number of animals in the population, etc.). The computer program scanned the data base, identified the most appropriate statistical method and performed the analysis.

Sublethal observations / clinical signs:

Table 1: Concentrations tested and corresponding percentage mortalities of bluegill (Lepomis macrochirus) exposed to CR-39

Nominal conc. (mg/L)	Cummulative % mortality
	24 h	48 h	72 h	96 h
1.7	100	100	100	100
1.0	30 (a,b,c)	100	100	100
0.6	0	0 (a,d)	30 (a,d,e,f)	40 (a,d,f)
0.36	0	10	10	10
0.22	0	0	0	0
Control	0	0	0	0

a) The fish appeared lethargic.

b) The fish exhibited darkened pigmentation.

c) Some fish exhibited muscle spasms.

d) Some fish were exophthalmic.

e) One fish was at the surface of the test solution.

f) Some fish exhibited a partial loss of equilibrium.

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 (and 95 % confidence interval) for bluegill exposed to CR-39, estimated by moving average angle analysis, was 0.57 (0.45-0.73) mg/L .

Executive summary:

Acute toxicity of the test substance to Bluegill (Lepomis macrochirus) was investigated according to "Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians" (U.S. EPA, 1975). Fish were exposed for 96 h to test substance concentrations of 0, 0.22, 0.36, 0.6, 1.0 and 1.7 mg/L. The 96-hour LC50 (and 95 % confidence interval) for bluegill exposed to CR-39, estimated by moving average angle analysis, was 0.57 (0.45-0.73) mg/L .

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1982-10-29 to 1982-11-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

Guideline study without GLP and analytics but acceptable for assessment.

Qualifier:

equivalent or similar to guideline

Guideline:

other: Test Protocol for Marine fishes, static, acute for Sheepshead minnow toxicity test, January 1982

Deviations:

yes

Principles of method if other than guideline:

Test was performed according to test protocol for marine fishes, static, acute for Sheepshead minnow toxicity test, January 1982.

GLP compliance:

no

Remarks:

Only raw data were stored

Analytical monitoring:

no

Remarks:

But due to good water solubility and slow hydrolysis the test item is expected to be stable for the duration of the test.

Vehicle:

yes

Test organisms (species):

Cyprinodon variegatus

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.707 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL=0.384-0.713

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

0.707 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL=0.384-0.713

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.976 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL=0.620-1.01

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

1.84 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL=1.80-1.87

Sublethal observations / clinical signs:

Table 1: Concentrations tested and corresponding percentage mortalities of Sheepshead minnow (Cyprinodon variegatus) exposed to CR-39 

Nominal conc. (mg/L)	Cummulative % mortality
	24 h	48 h	72 h	96 h
0.125	0	0	0	0
0.25	0	0	0	0
0.50		0	0	0
1.0	0	55	100	100
2.0	60	100	100	100
Control	0	0	0	0
Solvent control	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 (and 95 % confidence interval) for Sheepshead minnow (Cyprinodon variegatus) exposed to CR-39, estimated by moving average
angle analysis, was 0.707 (0.384 - 0.713) mg/L.

Executive summary:

Acute toxicity of the test substance to Sheepshead minnow (Cyprinodon variegatus) was investigated according to test protocol for marine fishes, static, acute for Sheepshead minnow toxicity test (January 1982). Fish were exposed for 96 h to test substance concentrations of 0, 0.125, 0.25, 0.50, 1.0 and 2.0 mg/L.

The 96-hour LC50 (and 95 % confidence interval) for Sheepshead minnow (Cyprinodon variegatus) exposed to CR-39, estimated by moving average angle analysis, was 0.707 (0.384 - 0.713) mg/L.

Description of key information

LC50 (96h) = 0.57 mg/L for Lepomis macrochirus (guideline equivalent or similar to EPA OPP 72 -1 (fish acute toxicity test); static)

LC50 (96h) = 0.707 mg/L for Cyprinodon variegatus (test was performed according to test protocol for marine fishes, static, acute for Sheepshead minnow toxicity test, January 1982)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

0.57 mg/L

Marine water fish

Marine water fish

Dose descriptor:

LC50

Effect concentration:

0.707 mg/L

Additional information

This endpoint is filled from data from two adequate fish toxicity tests (Sousa, 1982; Ward,1982b). The 96 hours LC50 values for 'diallyl 2,2'-oxydiethyl dicarbonate' in freshwater and saltwater species are 0.57 mg/L and 0.707 mg/L, respectively.