Registration Dossier
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EC number: 248-654-8 | CAS number: 27776-01-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to fish
Administrative data
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Study period:
- From the 20th February to the 9th April, 1987
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- This study is not considered a long-term study. In addition, adverse effects were observed at concentrations far above water solubility limit.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- yes
- Remarks:
- Details on test animals, system and test medium were not provided, no analytical monitoring.
- GLP compliance:
- yes
- Remarks:
- Reported only for the conservation of the study data and tested material inside the laboratory
Test material
- Reference substance name:
- Jarylec C
- IUPAC Name:
- Jarylec C
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
- Water solubility (under test conditions): 400 ppm
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was dissolved in acetone and then disolved in the assay water (constant volume: 1 mL of acetone/L of water). The solutions obtained are distributed in test containers (capacity 2 L) at a rate of 1 L/h.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- 20 fishes per test recipient were used for the test substance and negative control (with and without acetone) assays. 10 fishes per concentration were tested in the reference substance (potassium dichromate) assay.
Study design
- Test type:
- flow-through
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- None
Test conditions
- Hardness:
- no data
- Test temperature:
- 23 °C
- pH:
- Varied from 6.88 to 7.69 acoording to the concentration tested and the day of measurement.
- Dissolved oxygen:
- The concentration in dissolved oxygen measured at the end of the test was equal to at least 60% of the saturation (corresponding to 8.3 mg/L at 23°C), except for the vehicle control. The measured range in vehicle control was 4.2 to 5.7 mg/L; for the whole other test concentrations, the measured range was 4.7 to 6.8 mg/L.
- Salinity:
- Freshwater.
- Nominal and measured concentrations:
- Nominal tested concentrations: 1, 2.5, 3.5, 5, 10, 15, 20, 30 and 40 mg/L.
- Details on test conditions:
-
TEST CONCENTRATIONS
- Spacing factor for test concentrations: between 1.3 and 2
- Range finding study
- Test concentrations: 0, 1, 2.5, 5, 25, 50 (96 h)
- Results used to determine the conditions for the definitive study: LC0 = 2.5 mg/L and LC100 = 50 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 2.53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Mortality was observed above water solubility limit only
- Details on results:
- - Cumulative mortality at each concentration and for each recommended observation time if possible:
. 1 mg/L: 15% (cumulative mortality)
Day 11 : 15% (no observation on Week-end: Days 9 and 10)
. 2.5 mg/L: 25% (cumulative mortality)
Day 9 : 15%
Day 12 : 25% (no observation on Week-end: Days 10 and 11)
. 3.5 mg/L: 85% (cumulative mortality)
Day 4 : 55% (no observation on Week-end: Days 2 and 3)
Day 8 : 70%
Day 11 : 85% (no observation on Week-end: Days 9 and 10)
. 5 mg/L: 80% (cumulative mortality)
Day 4 : 20% (no observation on Week-end: Days 2 and 3)
Day 6 : 35%
Day 7 : 45%
Day 8 : 55%
Day 9 : 65%
Day 12 : 80% (no observation on Week-end: Days 10 and 11)
. 10 mg/L: 100% (cumulative mortality)
Day 1 : 50%
Day 4 : 100% (no observation on Week-end Day 3)
. 15 mg/L: 100% (cumulative mortality)
Day 1 : 65%
Day 2 : 100%
. 20 mg/L: 100% (cumulative mortality)
Day 1 : 90%
Day 2 : 100%
. 30 mg/L: 100% (cumulative mortality)
Day 2 : 100% (no observation reported on Day 1)
. 40 mg/L: 100% (cumulative mortality)
Day 1 : 100%
- Mortality in the controls:
No mortality in any control groups
- Behavioural observation of the fish:
No other toxicity effect other than mortality was observed in any animals. - Results with reference substance (positive control):
- - Results with reference substance valid? yes (according to French Guidelines AFNOR 90 303 - June 1985): LC50 at 24 h lies between 200 and 400 mg/L
- LC50: 259.9 mg/L - Reported statistics and error estimates:
- No statistics performed, graphical evaluation of the LC50 values.
Any other information on results incl. tables
Table 1: Fish mortality rate in the 14-day study
Tested Concentration [mg/L] | Mortality rate throughout the 14-d study period | |
Number of individuals (n/20) | % | |
Blank control | 0 | 0 |
Vehicle control | 0 | 0 |
1 | 3 | 15 |
2.5 | 5 | 25 |
3.5 | 17 | 85 |
5 | 16 | 80 |
10 | 20 | 100 |
15 | 20 | 100 |
20 | 20 | 100 |
30 | 20 | 100 |
40 | 20 | 100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- No mortality in control tests, measured dissolved oxygen always at least 60% of saturation, reference substance assay (only 24 h) considered valid
- Conclusions:
- The 14d-LC50 of the test item tested with Brachydanio rerio was determined to be 2.53 mg/L, which is far above water solubility limit of the test item.
- Executive summary:
In this prolonged acute toxicity test on fish performed according to the OECD Guidelines 204, 20 fishes (Brachydanio rerio) per concentration were exposed to JARYLEC C (Benzyl/dibenzyltoluene) during 14 days. The substance was administered to fish in test water after it had been dissolved in acetone. The resulting LC50 of JARYLEC C was determined to be 2.53 mg/L after 14 days. Mortality was observed at all concentrations tested, except in the control groups. At the lowest concentration tested (i.e. 1 mg/L), 3/20 animals died throughout the study period. All tested concentrations were above water solubility limit of the registered substance. Therefore, the results are considered irrelevant for regulatory purposes. This study is disregarded.
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