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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 30, 2014 until June 23, 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Version / remarks:
version adopted July 17, 1992
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Test material (Batch No. 03100048/310) provided by the sponsor Raschig GmbH
- Expiration date of the lot/batch: 12.04.2015
- Purity test date: not available

RADIOLABELLING INFORMATION (if applicable): not applicable

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stable
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: highly soluable in water, stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any): none
- Final dilution of a dissolved solid, stock liquid or gel: not applicable
- Final preparation of a solid: not applicable

FORM AS APPLIED IN THE TEST (if different from that of starting material): not applicable

OTHER SPECIFICS: none

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: activated sludge, micro organisms from a domestic waste water treatment plant
- Origin: sewage plant Neustadt, Germany
- Method of cultivation: The sludge was filtrated, washed twice with tap water, then washed with and re-suspended in test medium. It was aerated until use.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
51.7 mg/L
Based on:
test mat.
Remarks:
corresponds to 20.1 mg organic C/L
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: According to the OECD TG 301D (In solution b) CaCl2*2H2O was used and in solution d) 0.4g Na2EDTA were added).
- Additional substrate: none
- Solubilising agent (type and concentration if used): none
- Test temperature: 21.4 - 24.0 °C
- pH: 7.5 (on day 28)
- pH adjusted: none
- CEC (meq/100 g): no information available
- Aeration of dilution water: for 72 h
- Suspended solids concentration: 24.9 mg/L
- Continuous darkness: no information available
- Other: none

TEST SYSTEM
- Culturing apparatus: glass flasks (2000 mL)
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration with purified, CO2-scrubbed, moistened air
- Method used to create anaerobic conditions: not applicable
- Measuring equipment: CO2 was measured by IC using a carbon analyser (TOC multi N/C 2199S, Analytik Jena)
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: scrubber flasks
- Other: none

SAMPLING
- Sampling frequency: on days 0, 2, 4, 7, 9, 11, 14, 18, 23, 29
- Sampling method: sample volume of 1 mL
- Sterility check if applicable: not applicable
- Sample storage before analysis: not applicable
- Other: none

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes (using HgCl2)
- Toxicity control: Yes
- Other: none

STATISTICAL METHODS: according to OECD TG 301D with consideration of oxygen uptake caused by nitrification
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
The reference item aniline was sufficiently degraded (>60 %) after 14 days, and to approx 77 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
In the toxicity control containing both, the test item and the reference item, a biodegradation of 35% was observed after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.
% Degradation
Key result
Parameter:
% degradation (CO2 evolution)
Value:
1
Sampling time:
29 d
Remarks on result:
not determinable

Any other information on results incl. tables

In a study according to OECD TG 301 B under GLP, the substance was found to be not readily biodegradable

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Executive summary:

The test substance was investigated for its ready biodegradability in a study according to OECD TG 301 B under GLP. The biodegradation was followed by the CO2-evolution. As a reference item aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.

The substance emitted almost no CO2, so that the biodegradation after 29 days was determined as 1%.

The reference item aniline was sufficiently degraded  (>60 %) after 14 days, and to approx. 77 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.

In the toxicity control containing both, the test item and the reference item, a biodegradation of 35% was observed after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.

In conclusion, the substance was not biodegradable.