Trimethyl-3-[(1-oxoallyl)amino]propylammonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diallyldimethylammonium chloride

EC Number:

230-993-8

EC Name:

Diallyldimethylammonium chloride

Cas Number:

7398-69-8

IUPAC Name:

N-allyl-N,N-dimethylprop-2-en-1-aminium chloride

Details on test material:

- Name of test material (as cited in study report): Dimethyldiallylammoniumchlorid ca.60%
- Molecular formula (if other than submission substance): C8H16N.Cl
- Molecular weight (if other than submission substance): 161.68
- Smiles notation (if other than submission substance): C(=C)CN{+}(c)(c)(.Cl{-})CC=C
- InChl (if other than submission substance): 1/C8H16N.ClH/c1-5-7-9(3,4)8-6-2;/h5-6H,1-2,7-8H2,3-4H3;1H/q+1;/p-1
- Substance type: non-volatile, organic
- Physical state: liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: aqueous solution (at about 60%)
- Lot/batch No.: R 950 Partie 15
- Expiration date of the lot/batch:
- Stability under test conditions: stable
- Storage condition of test material: no data

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 6.25 and 100 m/l
- Sampling method: At the start of the test (0h), samples from vessels without daphnids, at the end of the test (after 48h) samples from vessels with and without daphnids.
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:

yes

Details on test solutions:

M4 water (according to ISO 10706)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain: Daphnia magna STRAUS 1820
- Source: in house breeding (sourced from Institut National de Recherche Chimique Appliquée, France in 1978)
- Age at study initiation: 2-24 hours (starting with the 3rd breed of parent animals)
- Feeding during test: No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

Hardness:

2.20-3.20 mmol/L

Test temperature:

20.3-20.4°C

pH:

8.0-8.2

Dissolved oxygen:

8.5-8.7 mg/L

Salinity:

no

Nominal and measured concentrations:

6.25, 12.5, 25, 50, and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom 20ml
- Type : open
- Material, fill volume: 10 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized water
- Total organic carbon: none
- Particulate matter: none
- Metals: none
- Pesticides: none
- Chlorine: none
- Alkalinity: 0.80-1.00 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: 550-650 µS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: artificial light16 h light/8 h dark
- Light intensity: about 2-7µE/(m2.s) at wave length of 400-700nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Swimming ability observed (visual) after 0, 3, 6 24 and 48 hours)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95%CL

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

DADMAC in aqueous solution at 60% concentration had no effect on the swimming ability of daphnia magna at a test concentration of 50 mg/l. The EC50 and EC 100 were both greater than 100mg/L. Therefore, the EC0 for DADMAC is 30 mg/l and the EC50 and EC100 are both greater than 60 mg/l.