Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Diallyldimethylammonium chloride
EC Number:
230-993-8
EC Name:
Diallyldimethylammonium chloride
Cas Number:
7398-69-8
IUPAC Name:
N-allyl-N,N-dimethylprop-2-en-1-aminium chloride
Details on test material:
- Name of test material (as cited in study report): Dimethyldiallylammoniumchloride ca 60%
- Molecular formula (if other than submission substance): C8H16N.Cl
- Molecular weight (if other than submission substance): 161.68
- Smiles notation (if other than submission substance): C(=C)CN{+}(c)(c)(.Cl{-})CC=C
- InChl (if other than submission substance): 1/C8H16N.ClH/c1-5-7-9(3,4)8-6-2;/h5-6H,1-2,7-8H2,3-4H3;1H/q+1;/p-1
- Substance type: non-volatile, organic
- Physical state: colourless liquid
- Composition of test material, percentage of components: 60% aqueous solution of Dimethyldiallylammonium chloride
- Analytical purity: no data given
- Impurities (identity and concentrations): no data given
- Lot/batch No.: 76334
- Expiration date of the lot/batch: no data given
- Stability under test conditions: satable
- Storage condition of test material: room temperture

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge : BASF laboratory wastewater plants treating municipal sewage
- Concentration of sludge: 30mg/L
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
54 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: BASF laboratory wastewater plants treating municipal sewage:
- Test temperature: room temperature
- pH: 7.4-7.7
- pH adjusted: no
- Aeration of dilution water: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 1000 ml vessels
- Number of culture flasks/concentration: 2 for test substance, 1 for the controls and the reference substance
- Method used to create aerobic conditions: no data
- Measuring equipment: no data
- Test performed in open system: no
SAMPLING
- Sampling frequency: Sample were taken for DOC analysis at days 0,1, 3, 5, 7, 10, 14, 17, 22, 24, 27, and 28
- Sampling method: no data
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: Degradation degree in the inhibition control after 14days
- Abiotic sterile control: Physico-chemical (abiotic) elimination of the test substance
- Toxicity control: no data
- Other: Elimination of the test substance by absorption
At day 14 of the test 1ml mercury chloride was added to the abiotic control assay
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
Validity criteria: The test is valid
Deviation of the degradation degree of the test substance in the plateau phase < 20%
Degradation degree of the reference substance > 70% after 14 days.
Degradation degree in the inhibition control > 35% after 14 days
% Degradationopen allclose all
Parameter:
% degradation (DOC removal)
Value:
>= 40 - <= 50
Sampling time:
28 d
Parameter:
% degradation (DOC removal)
Value:
ca. 0
Sampling time:
10 d
Details on results:
Degradation degree in the inhibition control after 14days: 40-50 %DOC
Physico-chemical (abiotic) elimination of the test substance: <10 % DOC
Elimination of the test subtance by absorption: <10 % DOC after 5 days

BOD5 / COD results

Results with reference substance:
Degradation % DOC 14 days = 90-100

Any other information on results incl. tables

DOC Decrease %

 Day  RS IH  PC   AC TS1  TS2   TS Mean value
0 0 0 0 0
1 2 -1 -4 -4  -2  -2  -2
3 25 3 -12 -13  -6  -12  -9
5 94 46 -9 -10  -5  -6  -6
7  98 42 -17   -10  -12  -11 
10 96 45 -9   -4  -9  -7 
14 96 47 -9   -2  -1 
17 93 49 -9   38  28  33 
22 97 73 0   46  45  46 
24 95 71 -9   43  40  42 
 27 99 70 -14   41  43  42 
 28 97 69 -13   45  43  44 

RS = reference substance, IH = inhibition control, PC = abiotic control, AC = absorption control, TS = test substance

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Degradation of the test substance was 40-50% (but less than 70%) measured by DOC removal in 28 days. Consequently dimethyldiallylammonium chloride is not readily biodegradable according to the OECD criteria.