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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Great waterflea
- Source: Originally obtained in 1978 from Institut National de recherche Chimique Appliquée, France. Since that date in-house cultured and bred
- Age at study initiation (mean and range, SD): < 24 hours (3rd brood progeny)
- Method of breeding: synthetic fresh water according to OECD 202, photoperiod: 16 hours light : 8 hours dark, 20 ± 1 °C culture temperature
- Feeding during test: no
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.48 mM
Test temperature:
19.5 - 19.8 °C
pH:
7.5 - 7.7
Dissolved oxygen:
8.6 - 9.0 mg/L
Nominal and measured concentrations:
nominal: control, 4.6, 10, 22, 46, 100 mg/L
mean measured: -, 4.5, 9.9, 22.2, 47, 98.5 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL glass beakers
- Material, size, headspace, fill volume: glass, 50 mL fill volume
- Renewal rate of test solution (frequency/flow rate): renewal period was 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 (OECD 202)
- Alkalinity: acid capacity up to pH 4.3: 0.8 - 1 mM
- Ca/Mg ratio: about 4:1
- Conductivity: 550 - 650 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: at 0 and 24 hours for initial test solution and 24 and 48 hours for renwed test medium

OTHER TEST CONDITIONS
- Photoperiod: without illumination

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
53.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
47 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
99 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50 (24 h): 1.1 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The 48 hour EC50 of the test material to Daphnia magna was determined to be 53.9 mg/l under the conditions of the test.
Executive summary:

In this guideline (OECD 202) study conducted with GLP certification, the 48 hour EC50 of the test material to Daphnia magna was determined to be 53.9 mg/l. The test was conducted under static conditions with test concentrations between 46 and 100 mg/l. The study result does not trigger classification as hazardous for the aquatic environment under the the conditions of the Classification, Labelling, and Packaging (CLP) regulation (1272/2008), as the test material is readily biodegradable and has a BCF <500.

Description of key information

 Study carried out according to guideline and with GLP compliance.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
53.9 mg/L

Additional information

In a semi-static test conducted according to OECD 202 guideline including analytical monitoring (BASF SE, 2010) an EC50 of 53.9 mg/L after 48 h based on mean measured concentration has been determined.