Hydroxycyclohexyl phenyl ketone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Mar. 22, 1990 to May 23, 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

No analytical monitoring. Tween 20 has been used as emulsifer

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Calculated amounts of the test substance and 60 mg Tween 20 per tank to produce the desired test concentrations were added to the water in each tank and were homogeniously distributed.
The test substance seemed to be homogeneously distributed in the test vessels for all concentrations and at the start and the end of exposure.
- Concentrations (nominal): 10, 18, 32 58 and 100 mg/L
- Controls: Blank (dechlorinated tap water) Vehicle (4 mg Tween 20 / L)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 20

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra-Fish
- Strain: Brachydanio rerio
- Source: West Aquarium / D-3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 29 mm (27 - 30 mm)
- Weight at study initiation (mean and range, SD): 0.23 g (0.21 - 0.26 g)
- Feeding during test: No
- Lighting: Fluorescent light, 16 hours daily
- Aeration: none
- Tempreature: 30 to 24°C within +/-1°C

ACCLIMATION
- Acclimation period: 75 days

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

192 mg CaCO3 / L

Test temperature:

23ºC

pH:

8.1 - 8.2

Dissolved oxygen:

86 - 100%

Salinity:

Freshwater

Nominal and measured concentrations:

Nominal: 10, 18, 32, 58 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria of 20 liters filled with 15 liters. (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Intervals of water quality measurement: oxygen content, pH and temperature were measured at 0, 24, 48, 72 and 96 hours.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: Fluorescent light
- Temperature: 20 to 24°C +/- 1°C

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortalities were recorded at 24, 48, 72 and 96 hours. Symptons of toxicity were observed daily.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

24 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL 20 - 28 mg/L; calculated

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: At 18 mg/L swimming behaviour and loss of equilibrium were recorded as moderate symptoms.
- Mortality of control: 0% mortality in blank or vehicle controls.

Reported statistics and error estimates:

The LC50 values were calculated according to BERKSON, JASA 48 (1953), 569-599.
The LC-values were graphically determined on gausso-logarithmic probability paper.

Sublethal observations / clinical signs:

	Effect concentration [mg/L]
	24 h	48 h	72 h	96 h
LC50	24	24	24	24
LC50 (graphically determined)				24

Conclusions:

The LC50 (96h) of the test substance is 24 mg/L (nominal concentration).

Executive summary:

In this guideline (EU C.1) study, the 96 hour LC50 to fish for the test material (EC 213-426-9) was determined to be 24 mg/l. The test was conducted in Danio rerio (previous name: Brachydanio rerio) under static conditions (freshwater), with test concentrations ranging from 10 to 100 mg/l. The study result does not trigger classification as hazardous for the aquatic environment under the the conditions of the Classification, Labelling, and Packaging (CLP) regulation (1272/2008), as the test material is readily biodegradable and has a BCF <500.

Description of key information

Study conducted to recognised testing guideline.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

24 mg/L

Additional information

In a toxicity study on fish according to the Official Journal of the European Communities L251, vol.27, C-01, 19/09/1984 (CIBA-GEIGY Ltd., 1990), the toxicity of the test item was determined. Zebra fish were used as test organisms and the LC50 (96 h) determined in the test was 24 mg/L.