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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guidelines
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Animal species: Zebra fish(Danio rerio)
Animal breeder/ supplier: Koyal aquarium,Nagpur.
Body length average: 1.73 cm
Body weight: 0.422g (average)

Acclimatization: Fishes, to be used in the study, are kept in the test water for seven days immediately before testing under following conditions:
Photoperiod: 16 hours light and 8 hours dark
Temperature range: 24.5 °C
pH range: 7.65
DO range: 7.7 mg/l
Feeding: Daily once
Aeration: Continuous
Mortality: (%) During last 48 hours = 0
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
150.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.05
Dissolved oxygen:
7.8 mg/l
Salinity:
No data
Conductivity:
No data
Nominal and measured concentrations:
0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L
Details on test conditions:
Photoperiod: 16 hours light and 8 hours dark
Volume of water: 2 liters
No. of animals per vessel: 8 fishes
Test temperature: 23 °C
Adaptation: Test conditions corresponded to housing conditions
Test dates: Test started at : 29-05-2017
Test Exposure date: 05-06-2017
Date of experimental termination : 09-06-2017
Withdrawal of food: 1 day before and during exposure
Aeration: Aeration in test vessels was provided 1 day before the start of experiment
Test vessels: The test vessels used were all plastic aquaria having the water capacity of 5liters.
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.312 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.625 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.2654-57-1 on Zebra fish(Danio rerio)was found to be 0.625 mg/L.

Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3 h

6 h

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.3125

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

2

2

2

2

1.25

4

4

-

-

-

-

-

-

4

4

4

4

4

4

2.5

4

4

-

-

4

4

4

4

4

4

4

4

4

4

5

4

4

-

-

4

4

4

4

4

4

4

4

4

4

 

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Symptom (cumulated) after

3 h

6 h

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.3125

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.625

4

4

M4

M4

M4

M4

M4

M4

M4

M4

M2

M2

M2

M2

1.25

4

4

M4

M4

M4

M4

M2

M2

-

-

-

-

-

-

2.5

4

4

IS4

IS4

-

-

-

-

-

-

-

-

-

-

5

4

4

IS4

IS4

-

-

-

-

-

-

-

-

-

-

 

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

 

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

8.23

8.25

8.31

8.32

8.31

8.32

8.32

8.31

0.3125

4

4

8.13

8.15

8.27

8.29

8.27

8.30

8.30

8.35

0.625

4

4

8.17

8.20

8.30

8.35

8.35

8.36

8.41

8.44

1.25

4

4

8.21

8.25

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

5

4

4

-

-

-

-

-

-

-

-

 

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/L) after

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.9

6.9

6.9

6.9

6.9

6.8

6.8

6.8

0.3125

4

4

6.9

6.9

6.8

6.8

6.7

6.7

6.7

6.7

0.625

4

4

6.9

6.9

6.8

6.8

6.7

6.7

6.7

6.7

1.25

4

4

6.9

6.9

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

5

4

4

-

-

-

-

-

-

-

-

 

Test temperature:

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.2

23.2

23.5

23.5

23.5

23.5

23.6

23.6

0.3125

4

4

23.2

23.2

23.5

23.5

23.5

23.5

23.6

23.6

0.625

4

4

23.2

23.2

23.5

23.5

23.5

23.5

23.6

23.6

1.25

4

4

23.2

23.2

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

5

4

4

-

-

-

-

-

-

-

-

 

 

Validity criteria fulfilled:
yes
Conclusions:
A 96 h study was conducted for test material on Zebra fish (Danio rerio), at a test concentrations of 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was determined to be 0.625 mg/L
Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L,  0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be 0.625 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L,  0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be 0.625 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.625 mg/L

Additional information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L,  0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be 0.625 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.