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Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was not easily soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 24 hour stirring. After the completion of 24 hours stirring, the stock solution was analytically detected. The water solubility obtained from the stock was then used for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be> 6.25 mg/L but < 12.5 mg/Lmg/l . Based on the LC50, it can be consider that the chemical was toxic and can be classified as aquatic chronic 3 as per the CLP classification criteria.

Short term toxicity to aquatic invertebrate:

The short-term toxicity of the test substance to aquatic invertebrate is predicted using EPI Suite ECOSAR version 1.10. On the basis of effects observed in a static freshwater system during a 48 hr exposure, the effect concentration (EC50) for the substance is estimated to be  14.820 mg/L. Based on this value, it can be concluded that the test chemical can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic 3 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 100 g/l  was prepared by dissolving colourless liquid  in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration used were12 , 17 , 24 , 33 , 46 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substance , in algae was determined to be 22.9 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to microorganism:

In different studies, the given test chemical, has been investigated for toxicity to microorganisms to a greater or lesser extent. The studies are summarized as below –

 

In the first key studyevaluation of toxicity of test chemical on Staphylococcus aureus toxicity to micro-organisms test was carried for 24 h under static condition.An overnight culture of each organism S. aureus was prepared. The 0.1 ml of organism was taken into 9.9 ml of sterile distilled water (SDW) to give 10 ml of 1:100 (10 ) dilution. The stock was maintained on nutrient agar slant and sub-cultured in nutrient broth for incubation at 37 °C prior to each antimicrobial testing. Inoculation of the test organisms on nutrient agar-prepared plates was achieved by flaming a wire loop on a spirit lamp, cooling the wire loop (air cooling) and fetching the test organisms.The discs were prepared using a Grade No. 1 Whatman filter paper. One hundred discs were obtained by punching and putting in vial bottles and sterilizing in an oven at 150 °C for 15 min. Thereafter the cups (9 mm diameter) were aseptically bored into the solid nutrient agar using a sterile cork-borer. The test solution of Methyl salicylate was introduced .The plates were left at room temperature for 2 h, allowed to diffuse into the medium, turned upside-down and thereafter incubated at 37 °C for 24 h in an incubator. The minimum inhibition concentration (MIC) of test chemical on Staphylococcus aureus was observed to be 200mg/l.

First study was supported by the second study from peer reviewed journal. To evaluate antibacterial activity of  test chemical on Activated sludge toxicity to micro-organisms test was carried out according to the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. The test was carried for 3 h under static condition. Test chemicalwas used as a test material to evaluate toxicity to microorganisms as per OECD guideline 209. Activated sludge was used as the test culture at pH 7.5 for 3 h The EC50 value on the basis of respiration inhibition test was observed to be >1000 mg/l.

Thus based on the above effects chemical toxicity value ranges from 200 to > 1000 mg/l.

 

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was not easily soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 24 hour stirring. After the completion of 24 hours stirring, the stock solution was analytically detected. The water solubility obtained from the stock was then used for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be> 6.25 mg/L but < 12.5 mg/Lmg/l . Based on the LC50, it can be consider that the chemical was toxic and can be classified as aquatic chronic 3 as per the CLP classification criteria.

Short term toxicity to aquatic invertebrate:

The short term toxicity of test material on aquatic invertebrate was evaluated based on the predicted data for test chemical along with data for study reports of structurally similar read across substances.

In the predication , the short-term toxicity of the test substance to aquatic invertebrate is predicted using EPI Suite ECOSAR version 1.10. On the basis of effects observed in a static freshwater system during a 48 hr exposure, the effect concentration (EC50) for the substance is estimated to be  14.820 mg/L. Based on this value, it can be concluded that the test chemical can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic 3 as per the CLP classification criteria.

The above prediction was supported by data of structurally similar read across substance ,determination of the inhibition of the mobility of daphnids was carried out with the substance Methyl 2-naphthyl ether according to OECD Guideline 202. The stock solution (200 g/L) was prepared by dissolving white powder in DMSO. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 5, 10, 20, 30, 40, 80 mg/L and the immobilisation effects were observed for 48 hours. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 26 mg/L on the basis of mobiity inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrates can can be classified as Aquatic chronic category 3 as per the CLP criteria.

 

For another structurally similar read across substance ,aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 100 g/l was prepared by dissolving white powder in acetone. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.1.2 , 2.5 , 5.0 , 10.0 , 20.0 , 40.0 mg/lconcentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 48.1 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 100 g/l  was prepared by dissolving colourless liquid  in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration used were12 , 17 , 24 , 33 , 46 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substance , in algae was determined to be 22.9 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to microorganism:

In different studies, the given test chemical, has been investigated for toxicity to microorganisms to a greater or lesser extent. The studies are summarized as below –

 

In the first key studyevaluation of toxicity of test chemical on Staphylococcus aureus toxicity to micro-organisms test was carried for 24 h under static condition.An overnight culture of each organism S. aureus was prepared. The 0.1 ml of organism was taken into 9.9 ml of sterile distilled water (SDW) to give 10 ml of 1:100 (10 ) dilution. The stock was maintained on nutrient agar slant and sub-cultured in nutrient broth for incubation at 37 °C prior to each antimicrobial testing. Inoculation of the test organisms on nutrient agar-prepared plates was achieved by flaming a wire loop on a spirit lamp, cooling the wire loop (air cooling) and fetching the test organisms.The discs were prepared using a Grade No. 1 Whatman filter paper. One hundred discs were obtained by punching and putting in vial bottles and sterilizing in an oven at 150 °C for 15 min. Thereafter the cups (9 mm diameter) were aseptically bored into the solid nutrient agar using a sterile cork-borer. The test solution of Methyl salicylate was introduced .The plates were left at room temperature for 2 h, allowed to diffuse into the medium, turned upside-down and thereafter incubated at 37 °C for 24 h in an incubator. The minimum inhibition concentration (MIC) of test chemical on Staphylococcus aureus was observed to be 200mg/l.

First study was supported by the second study from peer reviewed journal. To evaluate antibacterial activity of  test chemical on Activated sludge toxicity to micro-organisms test was carried out according to the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. The test was carried for 3 h under static condition. Test chemicalwas used as a test material to evaluate toxicity to microorganisms as per OECD guideline 209. Activated sludge was used as the test culture at pH 7.5 for 3 h The EC50 value on the basis of respiration inhibition test was observed to be >1000 mg/l.

Thus based on the above effects chemical toxicity value ranges from 200 to > 1000 mg/l.

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