m-toluidine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Concept NEN reports 6501 from the Dutch Standard Organization (1980)

Principles of method if other than guideline:

additionaly: Canton et al. (1975)
NEN 6501 (1980), Determination of acute toxicity with Daphnia magna. Dutch Standardization Organization, Rijswijk, The Netherlands

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

-Only the lowest and highest tested concentrations were measured by GC

Vehicle:

not specified

Details on test solutions:

- were prepared in "Dutch Standard water", (DSW, Canton and Sloff, 1982)

Test organisms (species):

Daphnia magna

Details on test organisms:

-Age at study initiation: <1d

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

ca. 1 mmol/L

Test temperature:

18-20°C

Nominal and measured concentrations:

ratio of concentrations: 3.2

Details on test conditions:

-test volume per vessel: 1 L
-number of replicates per concentration: 2
-25 daphnids per vessel

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.73 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: corrected for measured recoveries

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.75 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Based on analytical measurements, the substance was shown to be stable over the test duration. Therefore, nominal concentrations can be used.

Validity criteria fulfilled:

not specified

Remarks:

Since the test is poorly reported the validity criteria cannot be assessed sufficiently. Nevertheless the test is considered as valid based on the data given

Conclusions:

For the substance a 48h-LC50 of 0.75 mg/L was determined on Daphnia magna.

Executive summary:

The short-term toxicity of m-toluidine towards invertebrates was tested using Daphnia magna yielding a 48h-LC50 of 0.75 mg/L related to nominal concentrations. The used guideline was according to the Concept NEN reports 6501 from the Dutch Standard Organization (1980) (Hermens, 1984).

Description of key information

For the substance a 48d-LC50 of 0.75 mg/L was determined on Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.75 mg/L

Additional information

Several tests on acute toxicity towards aquatic invertebrates are available. According to the Guidance on information requirements and chemical safety assessment R.7b (ECHA, 2012) it is recommended not to take tests with an exposure duration of 24 hours into account for assessment as these values have considerable variability in the repeatability of its results.

The short-term toxicity of the substance towards invertebrates was tested using Daphnia magna yielding a 48h-LC50 of 0.75 mg/L related to nominal concentrations. The test concentrations were confirmed by analytical monitoring. The used guideline was according to the Concept NEN reports 6501 from the Dutch Standard Organization (1980).

Another test on the acute toxicity of m-toluidine to the invertebrate Daphnia magna was performed according to the Dutch Standardization Organization Method NEN 6501. For a test period of 48 hours a LC50 value of 0.15 mg/l was reported. The author reported in the test the mortality occurred over a wide range of concentrations. It is assumed that no confidence limit could be given due to mathematical reasons. Although this was the most sensitive short-term result it was not taken into account for assessment as it shows very high uncertainty.

One more low acute toxicity result towards Daphnia magna obtained in a reproduction test is available. The test was conducted by the National Institute of Technology and Evaluation, Japan. The result is not assignable and is therefore not taken into account for assessment. This is in line with the outcome of data evaluation within the scope of the ICCA/HPV-program. Preparing the SIDS dossier the result is also not considered for assessment.