Registration Dossier
Registration Dossier
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EC number: 214-230-6 | CAS number: 1115-70-4
Endpoint summary
Administrative data
Description of key information
Aquatic toxicity of metformin HCl
| Study Type |
Organism |
Effect concentation |
| Acute toxicity (TAD 4.08) |
Daphnia magna | 48h-NOEC: 78 mg/L;48h-EC50: 130 mg/L |
| Acute toxicity (EU C.2) |
Daphnia magna | 48h-EC50: 64 mg/L (literature study, supportive information) |
| Acute toxicity (TAD 4.11) | Lepomis macrochirus | 96h-NOEC: 982 mg/L; 96h-LC50: >982 mg/L |
| Chronic toxicity (OECD 211) | Daphnia magna | 21d-NOEC: ≥32 mg/L |
| Chronic/Acute toxicity Algae (EU C.3) | Desmodesmus subspicatus | 72h-EC50: >320 mg/L |
| Chronic toxicity (ISO /WD20079) | Lemna minor | 7d-EC50: 110 mg/L |
| Chronic toxicity (OECD 210) | Danio rerio | 34d-NOEC: ≥12 mg/L |
In general, metformin HCl has a very low hazardous potential on
aquatic organisms. In acute aquatic toxicity tests, metformin HCl did
not cause any adverse effects to fish (Lepomis macrochirus) and only
weak effects to invertebrates (Daphnia magna). Chronic toxicity testing
with aquatic organisms also resulted in either no (Desmodesmus
subspicatus, Daphnia magna, Danio rerio) or weak (Lemna minor) adverse
effects at the concentrations tested. The lowest NOEC from aquatic
toxicity studies was the 34d-NOEC of ≥12
mg/L obtained in the Early life stage test with Danio rerio.
Tests on Microbial Inhibition
| Study Type |
Organism |
Effect concentation |
| Microbial growth inhibition (FDA TAD 4.02) |
Azotobacter chroococcum | MIC: 800 mg/L |
| Aspergillus clavatus | MIC: >1000 mg/L | |
| Penicillium canescens | MIC: >1000 mg/L | |
| Chaetomium globosum | MIC: >1000 mg/L | |
| Pseudomonas fluorescens | MIC: >1000 mg/L | |
| Anabaena flos-aquae | MIC: 100 mg/L | |
| Bacillus megaterium | MIC: >1000 mg/L | |
| Respiration inhibition | Activated sludge | 3-hours EC50: 233 mg/L |
| DOC removal | Activated sludge | 24-hours EC50: >250 mg/L |
| Nitrification inhibition | Activated sludge | EC50: 48-65 mg/L |
Studies on microbial growth inhibition, respiration inhibition and
nitrification inhibition did not result in any inhibition at
concentrations below 100 mg/L.
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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