Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004-05-25 to 2004-08-31
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid: viscous
Details on test material:
No data

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Not applicable

Test solutions

Vehicle:
no
Details on test solutions:
The mother solution preparation is carried out at a concentration of 100 mg/L.
The mother solution is diluted in order to obtain a concentration range that permit to calculate the EC50-72 hours; the tested concentrations are 10; 25; 50; 75; 100 mg/L

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Species: Selenastrum capricornutum ATCC 22622
Source: Culture collection of Alage and Protozoa-Cumbria U.D., CCAP n°27814
In cultivation in the laboratoy.

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
No post exposure observation period

Test conditions

Hardness:
No data
Test temperature:
23°C +/- 2°C
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
Nominal concentrations are 10; 25; 50; 75; 100 mg/L
Details on test conditions:
The test solution has to be prepared before adding algae.
At the same time that the control, we have to test concentrations forming a geometrical series that allows to obtain 100% growth inhibition after 72 hours and a series of intermediary inhibitions permitting to calculate EC50-72 hours.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 76 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Results with reference substance (positive control):
No data

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
After a 72 hours incubation, a growth inhibition of 50% of Selenastrum capricornutum appears for a 76 mg/L concentration.
EC50 72h Selenastrum capricornum = 76 mg/L
Executive summary:

Determination, in the conditions defined in this norm, of growth inhibition observed after 24, 48 and 72 hours.

EC50 -72 hours is obtained by calculation.

After a 72 hours incubation, a growth inhibition of 50% of Selenastrum capricornutum appears for a 76 mg/L concentration. EC50 72h Selenastrum capricornum = 76 mg/L