Acylation product between cocoyl chloride and aminoacids

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004-03-12 to 2004-06-09

Reliability:

2 (reliable with restrictions)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

no

Details on test solutions:

The mother solution preparation is carried out at a concentration a little bit higher than 100 mg/L.
Dilutions are made in order to obtain concentrations in a geometrical series; that permit to calculate the EC50-48 hours; the concentrations are; 0.6; 1.1; 5.6; 11; 56 and 111 mg/L.
The test item solution has to be prepared immediately before the introduction of Daphnia's.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus aged more than 6 hours and less than 24 hours at the beginning of the test.
Source: U.G. Gent University_laboratory Prof.Persoone. In incubation in the laboratory
Alimentation: Cultivation of Selenastrum capricornutum algae

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no post exposure observation period

Hardness:

250 mg/l (in CaCO3)

Test temperature:

21°C ± 1°C.

pH:

pH 7.6 (± 0.2)

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

Nominal concentrations: 0.6; 1.1; 5.6; 11; 56 and 111 mg/L.

Details on test conditions:

20 organisms by test concentration (2 parts of 10).

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

no data

Results:

Concentrations	Immobile Daphnia’s after 48 hours		% immobility
	A	B
Blanc	0	0	0
0.6	0	0	0
1.1	0	0	0
5.6	0	1	10
11	2	2	40
56	5	6	55
111	10	9	95

Calculation method: Probit method

Validity criteria fulfilled:

not specified

Conclusions:

After a 48 hours incubation, the immobilisation of 50% Daphnia’s magna appears for a 45 mg/l
concentration.
EC50 48h Daphnia magna : 45 mg/l

Executive summary:

Determination, in the conditions defined in this norm, of the initial concentration (it means the concentration at the beginning of the test) that, in 24 and 48 hours, immobilises 50 % of the Daphnia’s put in experimentation.

This concentration called effective initial inhibitive concentration is designed by EC50-24/48 h.

After a 48 hours incubation, the immobilisation of 50% Daphnia’s magna appears for a 45 mg/l

concentration.

EC50 48h Daphnia magna : 45 mg/l

Description of key information

After a 48 hours incubation, the immobilisation of 50% Daphnia’s magna appears for a 45 mg/l concentration.
EC50 48h Daphnia magna : 45 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

ca. 45 mg/L