Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP compliant study conducted according to OECD Guidelines, with no or minor deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
Name: Linalool Oxide
Batch No.: PE00097239
Purity: 98.5 % (Sum of cis- and trans- isomers)
Expiry date: 2016-08-27

Please note that although the substance is described in the report with the IUPAC name: 2-(5-methyl-5-vinyltetrahydrofuran-2-yl)propan-2-ol related to the CAS number 1365-19-1, recent analytical data had been determined the substance to be a multi-constituent 2-(tetrahydro-5-methyl-5-vinyl-2-furyl)propan-2-ol related to the CAS number 60047-17-8.

As the chemical manufacturing process has not changed since the report was written, the recent analytical information is relevant to the batch which was tested.

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Five concentrations were tested in a geometrical series with a dilution factor of 1.2 (nominal): 96.0 -116-139 -167 - 200 mg/L. Three replicates were tested for each test item concentration and six replicates for the control.

The concentrations were selected based on the results of a non-GLP preliminary range-finder.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Pseudokirchneriella subcapitata HINDÄK, SAG 61.81

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Nominal and measured concentrations:
Nominal: 96, 116, 139, 167, 200mg/L
Details on test conditions:
With regard to the volatility of the test item, closed bottles without headspace were used to reduce losses of the test item.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
178 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: (154-200)
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
116 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
The measured concentrations of Linalool Oxide in the fresh media (0 h) were in the range of 102 to 119 % of the nominal values at all tested concentration levels. The measured concentrations in the old media (72 h) were in the range of 93 to 110 % of the nominal values of all concentrations.
Therefore, all effect values given are based on the nominal test item concentrations of Linalool Oxide.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 72 h-ErC50 for Algae was > 200 mg/L in the test conditions. The corresponding NOEC and EC10 based on growth rate inhibition were 116 and 178 mg/L respectively.