7-anilino-4-hydroxynaphthalene-2-sulphonic acid

Endpoint summary

Additional information

Short-term toxicity to fish:

Predicted model data and experimental study result for the test chemical 7-Anilino-4-hydroxynaphthalene-2-sulphonic acid (CAS No. 119-40-4) with respective read across were reviewed to summarize the following information:

In key study the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours LC50 was estimated to be 2917.242 mg/l on fish for substance 7-Anilino-4-hydroxynaphthalene-2-sulphonic acid (CAS no. 119-40-4) with mortality effects.

Above predicted data supported by experimental study from GSBL – Joint Substance Data Pool of the German Federal Government and the German Federal States; 2015 which indicate based on effects such as O2 consumption, growth, mortality and buoyancy, the 96 hrs LC0 value for test substance 7-Anilino-4-hydroxynaphthalene-2-sulphonic acid was found to be 100 mg/l.

From peer reviewed journal Chemophere. Vol. 28, No. 12, pp. 2203.2236, 1994 following studies available for different read across substances:

For read across chemical 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid (Cas no. 87-02-5) which is indicates lethal concentration (LC0) of test substance 7-amino-4-hydroxy-2-naphthalene-sulfonic acid for fish Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate was found to be 1000 mg/l for 96 hrs.

For read across chemical 7-hydroxy-1,3-naphthalene –disulfonic acid,dipotassium salt (Cas no. 842-18-2) which is indicates lethal concentration (LC0) of test substance 7-hydroxy-1,3-naphthalene –disulfonic acid,dipotassium salt for fish Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate was found to be >=1000 mg/l for 72 hrs.

For read across chemical 6-amino-4-hydroxy-2-naphthalene-sulfonic acid (Cas no. 90-51-7) which is indicates lethal concentration (LC0) of test substance 6-amino-4-hydroxy-2-naphthalene-sulfonic acid for fish Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate was found to be 1000 mg/l for 48 hrs.

Thus, based on above all lethal concentration values, it can be concluded that the target chemical 7-Anilino-4-hydroxynaphthalene-2-sulphonic acid (CAS No. 119-40-4) can be considered as non-toxic to fish and thus cannot be classified as hazardous as per the CLP criteria.

Short term toxicity to aquatic invertebrates:

Various predicted studies and read across study for the4-Hydroxy-7-(phenylamino)naphthalene-2-sulfonic acidwere reviewed to summarize the following information: 

48 hrs aquatic toxicity studies (SSS QSAR prediction model, 2016) were conducted to assess toxic effects of4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid (CAS no119-40-4)and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the effective concentration (EC50) for the4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid (CAS no 119-40-44)was estimated to be 135.234634399 mg/Lon the basis of intoxication. Based on the result the4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid was considered to be not classified as per CLP regulations.

48 hrs aquatic toxicity studies (EPI suite, ECOSAR version 1.1, 2016) were conducted to assess toxic effects of the 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid (CAS no 119-40-4) and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for 4-Hydroxy-7-(phenylamino)naphthalene-2-sulfonic acid (CAS no119-40-4) was estimated to be 1486.686mg/l .Based on the result 4-Hydroxy-7-(phenylamino)naphthalene-2-sulfonic acid was considered as not classified according to the CLP regulations.

 

The short term toxicity to aquatic invertebrates test (ECOTOX: Aquatic Report, U.S environmental Protection agency, 2016) was performed in 5 daphnia magna with age below 24 h. The temperature and pH of chemical was 20 degree C and 7.4.Freshwater medium was used in static condition for 48 hrs. Intoxication was the basis of effects. After experiment the EC 50 value for short term toxicity to aquatic invertebrates for Chlortetracycline (57-62-5) was determined to be 400 mg/l.

 

Using the same reference database for read across chemical Oxytetracycline hydrochloride, the short term toxicity to aquatic invertebrates test (ECOTOX: Aquatic Report, U.S environmental Protection agency, 2016) was performed in 5 daphnia magna with age below 24 h. The temperature of chemical was 21 degree C. Freshwater medium was used in static condition for 48 hrs. Intoxication was the basis of effects. After experiment the EC50 value for short term toxicity to aquatic invertebrates for Oxytetracycline hydrochloride (2058-46-0) was determined to be 250 mg/l.

 

As based on the above predicted data and read across study, it can be considered that 4-Hydroxy-7-(phenylamino)naphthalene-2-sulfonic acid (CAS no 119-40-4) is not likely to be toxic to aquatic invertebrates and can be considered to be not classified as per CLP regulation.

Toxicity to aquatic algae and cyanobacteria

Predicted data for the target chemical 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid (CAS No. 119-40-4) and the study for its read across substance were reviewed to summarize the following information:

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours EC50 was estimated to be 708.246 mg/l on green algae for substance 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid with growth inhibition effects.

The effect of test item disodium 4-amino-5-hydroxynaphthalene-1,7-disulphonic acid, CAS No. 130-23-4 was studied [UERL Study Report, Sustainability Support Services (Europe) AB (Report no. 130-23-4/01/2016/AT), 2016] on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200mg/l.

 

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 4-Amino-5-hydroxynaphthalene-1,7 -disulp honic acid according to OECD Guideline 201 [ABITEC study report, Sustainability Support Services (Europe) AB (study report no. 506/16,2016 ]. The stock solution (200 mg/l) was prepared by dissolving light grey powder in OECD growth medium. Test solutions of required concentrat

ions were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 2.2, 11, 25, 55 and 120 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 4-Amino-5-hydroxynaphthalene-1,7-disulphonic acid in Desmodesmus subspicatus was determined to be 129.4 mg/L on the basis of effects on growth rate in a 72 hour study.

 

Based on the overall reported results for target and its read across substance, it can be concluded that the substance 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.