Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study according to OECD guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

EC Number:

228-726-5

EC Name:

Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

Cas Number:

6337-43-5

Molecular formula:

C22H26O8

IUPAC Name:

1,3-diethyl 2-({4-[3-ethoxy-2-(ethoxycarbonyl)-3-oxoprop-1-en-1-yl]phenyl}methylidene)propanedioate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

other: test vessels were shaken during exposure

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 0.007 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.007 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Appearance of test solution:

The solution of 0.00743 mg/L exposure level was white suspension at the start of the exposure, and that at the end of the exposure was green white suspension due to the algea growth. In addition, white suspended substance was slightly observed on the surface, and white pellet was also found on the bottom at the start and end of the exposure.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item had no impact on test organisms at around the solubility of the test item in medium.

Executive summary:

The present study was conducted as a limit test to confirm whether the test item affected the test organisms at the solubility of the test item in medium or not. Although the measured concentrations of the test item in the test solution were slightly above the solubility of the test item in medium (125 ~ 137 %), those were kept within ± 20 % of those at the start of the exposure. In addition, as the environmental conditions were also within the suitable range, we evaluated that this study complied with the test guidelines.

As a result, the concentrations of the test item in the test solution were maintained at around the solubility of the test item in medium. In addition, no effect on the growth of the test organisms was observed under the test conditions. Therefore, it was concluded that the test item had no acute impact on test organisms at around the solubility of the test item in medium.