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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study according to OECD guideline and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report Date:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
other: test vessels were shaken during exposure
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.007 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.007 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

Appearance of test solution:

The solution of 0.00743 mg/L exposure level was white suspension at the start of the exposure, and that at the end of the exposure was green white suspension due to the algea growth. In addition, white suspended substance was slightly observed on the surface, and white pellet was also found on the bottom at the start and end of the exposure.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item had no impact on test organisms at around the solubility of the test item in medium.
Executive summary:

The present study was conducted as a limit test to confirm whether the test item affected the test organisms at the solubility of the test item in medium or not. Although the measured concentrations of the test item in the test solution were slightly above the solubility of the test item in medium (125 ~ 137 %), those were kept within ± 20 % of those at the start of the exposure. In addition, as the environmental conditions were also within the suitable range, we evaluated that this study complied with the test guidelines.

As a result, the concentrations of the test item in the test solution were maintained at around the solubility of the test item in medium. In addition, no effect on the growth of the test organisms was observed under the test conditions. Therefore, it was concluded that the test item had no acute impact on test organisms at around the solubility of the test item in medium.