Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study according to OECD guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

EC Number:

228-726-5

EC Name:

Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

Cas Number:

6337-43-5

Molecular formula:

C22H26O8

IUPAC Name:

1,3-diethyl 2-({4-[3-ethoxy-2-(ethoxycarbonyl)-3-oxoprop-1-en-1-yl]phenyl}methylidene)propanedioate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 1 °C

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Concentration of test item in test solution:
The measured concentrations of the test item in the test solution were 0.00461 and 0.00611 mg/L at the preparation (start of the exposure and after the renewal), and 0.00307 and 0.00406 mg/L (compared to the concentration at the preparation: 66.7 and 66.4 %) at 24 hours after (before the renewal and end of the exposure).

Any other information on results incl. tables

Calculating method of EC50:

The EC50 value was estimated as "> the test concentration" since no less than 50 % of immobility was not observed in the present exposure level. The results of the study were estimated by geometric mean of the measured concentrations as the test concentration.

Applicant's summary and conclusion

Conclusions:

Because no adverse effect was observed during the exposure period, it was decided that the test item has no potential for acute effect to test organisms at around the solubility of the test item in dilution water.

Executive summary:

Because no adverse effect was observed during the exposure period, it was decided that the test item has no potential for acute effect to test organisms at around the solubility of the test item in dilution water.