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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study according to OECD guideline and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Qualifier:
according to
Guideline:
ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Eisenia fetida

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d

Results and discussion

Effect concentrations
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Any other information on results incl. tables

Mortality:

No mortality occurred at the limit test item concentration of 1000 mg/kg soil DW after 14 days of exposure.

Behaviour and Pathological Symptoms:

Earthworms in the control and at the limit test item concentrations of 1000 mg/kg soil DW showed no changes in the behaviour or pathological symptoms after 14 days of exposure.

Live Weight of the Earthworms:

During the test the biomass loss of the earthworms was below 20 % in the control. Compared to the control the earthworms biomass was not statistically significantly reduced at the limit test item concentration of 1000 mg/kg soil DW. In contrast, the earthworm biomass at 1000 mg/kg soil DW was significantly enhanced.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No mortality as well as no pathological symptoms or changes in the earthworm behaviour were observed at the limit test item concentration of 1000 mg/kg soil DW after 14 days of exposure. Consequently, the No Observed Effect Concentration (NOEC) of the substance with respect to mortality, loss of body weight and other symptoms was determined to be >= 1000 mg/kg soil DW.
Executive summary:

No mortality as well as no pathological symptoms or changes in the earthworm behaviour were observed at the limit test item concentration of 1000 mg/kg soil DW after 14 days of exposure. Compared to the control the biomass of earthworms exposed to the substance at the test concentration of 1000 mg/kg soil DW was not statistically significantly reduced at test end. Consequently, the No Observed Effect Concentration (NOEC) of the substance with respect to mortality, loss of body weight and other symptoms was determined to be >= 1000 mg/kg soil DW.