Registration Dossier

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study according to OECD guideline and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report Date:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Sampling and analysis

Details on sampling:

Frequency ofanalysis
(1) Test water analysis
The test water of each level was analyzed once before first analysis of test fish and at the same time as analysis of test fish thereafter. The number of each sample was one.
(2) Test fish analysis
The test fish ofLevel 1 and 2 was analyzed five times in duration of exposure. Four fish were taken out at each sampling time and divided into two groups, then both were analyzed individually*3.
The control fish was analyzed before the experimental starting and after the experimental completion. Four fish were taken out at each sampling time and divided into two groups, and then each was analyzed individually. In addition, six fish were separately taken out and three groups (two fish per group) were used for measurement of lipid contents.
*3 Because one fish was not enough to the analysis sensitivity for the analysis of the test item, two fish were pooled for a group.

Test solutions

Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:

The test item and HCO- 40 (50 times amount of the test item) were dissolved in N,N-dimethylformamide with supersonic wave irradiation to prepare a stock solution.

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
Length 6.9 - 8.5 cm

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d

Test conditions

Hardness:
Total hardness (Ca, Mg) mg/L 13.2
Test temperature:
Level 1 24.2 - 24.5 °C
Level 2 24.1 - 24.5 °C
Control24.0 - 24.5 °C
pH:
Level 1 7.8 -7.9
Level 2 7.8 -7.9
Control 7.8 -7.9
Dissolved oxygen:
Level 1 7.8 - 8.0 mgIL
Level 2 7.8 mgIL
Control 7.4 -7.9 mgIL
Nominal and measured concentrations:
Level 1: 2µg/L
Level 2: 0.2 µg/L
Details on estimation of bioconcentration:
Since the test substance concentrations in fish were below the detection limit no BCF was calculated. Therefor

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
<= 2
Basis:
whole body w.w.
Calculation basis:
other:
Remarks on result:
other: On basis of the detection limit of the substance in fish a theoretical maximum BCF was determined
Remarks:
Conc.in environment / dose:2 µg/L
Type:
BCF
Value:
< 19
Basis:
whole body w.w.
Calculation basis:
other:
Remarks on result:
other: On basis of the detection limit of the substance in fish a theoretical maximum BCF was determined
Remarks:
Conc.in environment / dose:0.2 µg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The maximum BCF based on detection limit of of the test item is determined to be 2.0 (test concentration 2µg/L) and 19 (test concentration 0.2 µg/l). Therefore the test item has a very low potential for bioaccumulation
Executive summary:

The maximum BCF based on detection limit of of the test item is determined to be 2.0 (test concentration 2µg/L) and 19 (test concentration 0.2 µg/l). Therefore the test item has a very low potential for bioaccumulation