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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
2 rats per sex and dose / poorly documented
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
4-allylveratrole
EC Number:
202-223-0
EC Name:
4-allylveratrole
Cas Number:
93-15-2
Molecular formula:
C11H14O2
IUPAC Name:
1,2-dimethoxy-4-(prop-2-en-1-yl)benzene
Test material form:
liquid
Specific details on test material used for the study:
Purity of each material was checked in the Organic Chemicals Synthesis Laboratory, AEQI, in Beltsville by gas chromatography: > 98%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 150-240 g
- Fasting period before study: 16 hr before dosing.
- Housing: Individually in suspended wire-mesh cages.

Administration / exposure

Route of administration:
other: Directly into the stomachs with a hypodermic syringe that had a ball-tipped intubation needle.
Vehicle:
unchanged (no vehicle)
Doses:
From 600 to 3038 mg/kg bw
No. of animals per sex per dose:
2 rats per sex and dose.
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 179 mg/kg bw
Based on:
test mat.
95% CL:
927.9 - 1 430.1

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The oral LD50 was determined to be 1179 mg/kg bw in rats.
Executive summary:

An acute oral toxicity test was performed in Sprague-Dawley rats. Test item up to 3038 mg/kg bw was administered to two rats per sex and per dose, directly into the stomachs with a hypodermic syringe that had a ball-tipped intubation needle. The animals were observed for 14 days after exposure. The oral LD50 was determined to be 1179 (± 251.1) mg/kg bw in rats.