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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
poorly documented
GLP compliance:
not specified
Specific details on test material used for the study:
4-Allyl-1,2-dimethoxybenzene
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Nominal and measured concentrations:
10, 18, 32, 56, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Temperature: 20 ± 1 ºC
- Adjustment of pH: No.
- Photoperiod:16 hours light/ 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
38.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The 48h-EC50 was determined to be 38.2 mg/L (95% CL: 34.4-42.6 mg/L) in Daphnia magna.
Validity criteria fulfilled:
not specified
Conclusions:
The 48h-EC50 was determined to be 38.2 mg/L in Daphnia magna.
Executive summary:

An acute Daphnia magna immobilization test was performed according to the OECD Guideline 202. Five Daphnids per replicate were exposed up to 100 mg/L (20 Daphnids per dose) for 48 hours under static conditions. Test item concentrations were measured by HPLC analysis. The 48h-EC50 was determined to be 38.2 mg/L (95% CL: 34.4-42.6 mg/L) in Daphnia magna based on mobility.

Description of key information

Key study: Test method OECD 202. The 48h-EC50 was determined to be 38.2 mg/L in Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
38.2 mg/L

Additional information