4-allylveratrole

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

poorly documented

GLP compliance:

not specified

Specific details on test material used for the study:

4-Allyl-1,2-dimethoxybenzene

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Nominal and measured concentrations:

1.0, 2.2, 4.6, 10, 22, 46, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL
- Initial cells density: 1*10E4
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 1

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 24 hours
- Light intensity and quality: 4000-5000 Lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Growth rate and biomass

Reference substance (positive control):

not specified

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

18.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

9.35 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

The 72h-EC50 based on growth rate inhibition in green algae is 18.9 mg/L (95% CL: 17.1-20.9 mg/L).
The 72h-EC50 based on biomass inhibition in green algae is 9.64 mg/L (95% CL: 8.82-10.5 mg/L).
The 72h-NOEC based on growth rate inhibition in green algae is 9.35 mg/L.
The 72h-NOEC based on biomass inhibition in green algae is 2.06 mg/L.

Validity criteria fulfilled:

not specified

Conclusions:

The 72h-EC50 and 72h-NOEC in green algae based on growh inhibition rate was determined to be 18.9 mg/L and 9.35 mg/L respectively.

Executive summary:

An algae growth inhibition test was performed according to OECD Guideline 201 in Pseudokirchneriella subpicata. 10E4 green algae were exposed up to 100 mg/L test item (3 replicates per dose) 72 hours under static condicions.Test item concentration was measured by HPLC analysis. Growth inhibition rate and biomass inhibition rates were evaluated. The 72h-EC50 and 72h-NOEC in green algae based on growh inhibition rate was determined to be 18.9 mg/L (95% CL: 17.1 -20.9 mg/L) and 9.35 mg/L respectively. The 72h-EC50 and 72h-NOEC in green algae based on biomass inhibition rate was determined to be 9.64 mg/L (95% CL: 8.82 -10.5 mg/L) and 2.06 mg/L respectively.

Description of key information

Key study: Test method OECD 201. The 72h-EC50 and 72h-NOEC in green algae based on growh inhibition rate was determined to be 18.9 mg/L and 9.35 mg/L respectively.

Key value for chemical safety assessment

EC50 for freshwater algae:

18.9 mg/L

EC10 or NOEC for freshwater algae:

9.35 mg/L