1,4-bis(vinyloxy)butane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996-05-23 to 1997-04-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study conducted under GLP.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

Deviations:

no

GLP compliance:

yes

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-bis(vinyloxy)butane

EC Number:

223-437-0

EC Name:

1,4-bis(vinyloxy)butane

Cas Number:

3891-33-6

Molecular formula:

C8H14O2

IUPAC Name:

1,4-bis(ethenyloxy)butane

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: DR. K. THOMAE GMBH, BIBERACH, Germany
- Age at study initiation: Young adult animals of a comparable weight were used
- Weight at study initiation: males 180 - 183 g, females 169 - 171 g
- Fasting period before study: 16 hours prior to administration (but water ad libitum)
- Housing: Five animals per cage (type: stainless steel wire mesh cages, DK-III), no bedding
- Diet: KLIBA-LABORDIAET 343, KLINGENTALMUEHLE AG KAISERAUGST, SWITZERLAND, as libitum
- Water: tap water ad libitum
- Acclimation period: at least one week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24
- Humidity (%): 30 - 70
- Air changes (per hr): no data, however housing in fully air-conditioned rooms
- Photoperiod (hrs dark / hrs light): 12 h / 12 h

IN-LIFE DATES: From: 1996-05-14 (day of first administration, before at least one week acclimatisation) To: 1996-05-23 (day of last administration)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Remarks:

bidest

Details on oral exposure:

The aqueous test substance formulation corresponds to the physiological medium.
Form of administration: emulsion

VEHICLE
- Concentration in vehicle: 20 g / 100 mL
- Amount of vehicle: 10 mL
- Justification for choice of vehicle: aqueous formulation corresponds to the physiological medium
- Purity: bidest water was used

MAXIMUM DOSE VOLUME APPLIED: 2000 mg/ kg bw

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: A check for dead and moribund animals was made twice on workdays and once on the other days. Weighing of both groups on day 0, day 7; on day 13 (males) and day 14 (females).
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, impaired general state, poor general state, dyspnoea, apathy, staggering, piloerection, salivation, body weight

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male/female

Dose descriptor:

LD0

Effect level:

>= 2 000 mg/kg bw

Based on:

test mat.

Mortality:

None

Clinical signs:

other: Males only: impaired or poor general state, dysponea, apathy, staggering, salivation. These symptoms are considered to be unspecific toxicity symptoms. The animals appeared normal one day after administration.

Gross pathology:

No abnormalities were noted at necropsy of animals sacrificed at the end of the study.